双花颗粒调脂作用实验研究  

Experimental studies on blood lipid regulating effects of Shuanghua granules

作　　者：吴芳洲[1] 徐玲[1] 刘家兰[1] 徐晓玉[1] 

机构地区：[1]西南大学药学院重庆市药效评价工程技术研究中心,重庆400715

出　　处：《中国中药杂志》2011年第11期1492-1498,共7页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2006BAC01A16);重庆市"十一五"规划项目(104290-41000307);秀山县政府项目

摘　　要：目的:研究双花颗粒对正常小鼠及不同高脂血症动物模型血脂的影响。方法:分别采用蛋黄乳致急性高脂血症和Triton WR-1339诱导的内源性高脂血症小鼠模型及食饵性高脂血症大鼠模型,观察不同剂量双花颗粒对高脂血症的预防作用。结果:与空白组比较,双花颗粒5.6,11.3,22.5 g.kg-1能有效降低正常小鼠血清甘油三酯(TG)水平(P<0.05,P<0.01),对总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和肝指数无明显影响;双花颗粒5.6,11.3 g.kg-1显著降低蛋黄乳高脂血症小鼠血清TC水平,22.5 g.kg-1显著降低Triton WR-1339诱导的高脂血症小鼠血清低密度脂蛋白胆固醇(LDL-C)水平(P<0.05,P<0.01);双花颗粒能有效抑制高脂血症大鼠血清和肝脏TC,TG,LDL-C水平,升高HDL-C值,使血清超氧化物歧化酶(SOD)、脂蛋白脂酶(LPL)、肝脂酶(HL)、总脂酶(LA)活性升高,降低丙二醛(MDA)与游离脂肪酸(NEFA)含量,同时显著降低不同切变率下的全血黏度和全血还原黏度、血浆黏度、红细胞刚性(IER)和聚集(IEA)指数(P<0.05,P<0.01)。双花颗粒能在一定程度上减轻高脂饮食造成的脂肪肝损伤。与高脂模型组相比,双花颗粒各给药组大鼠粪便中脂质含量无显著增加。结论:双花颗粒能明显调节高脂血症小鼠或大鼠脂质代谢紊乱,其调脂机制不是通过增加脂质排泄来实现,可能与其抗脂质过氧化,改善血液流变性,提高LPL,HL,LA酶活性有关。Hyperlipidemia plays a vital role in cardiovascular disease,and threatens our lives.The aim of this paper is to study the effects of Shuanghua granules on blood lipid in normal mice and different hyperlipidemia models.Acute and endogenous hyperlipidemia was induced in mice with yolk and Triton WR-1339 respectively.The model of hyperlipidemia in rats was set up by feeding high cholesterol diet.Then preventive effects of Shuanghua granules was observed compared with lovastatin and Zhibituo.We found that Shuanghua granules 5.6,11.3,22.5 g·kg-1 could significantly reduce the serum TG level in normal mice（P0.01,P0.05）.No significant difference was found in liver index,serum TG and HDL-C levels.When the mice were treated with either yolk or Triton WR-1339 in the presence of Shuanghua granules,the plasma lipoprotein levels（TC and LDL-C） were significantly reduced（P0.01,P0.05）.Shuanghua granules could reduce the serum TC,TG,LDL-C,MDA,NEFA and liver TC,TG,LDL-C levels,simultaneously raise serum and liver HDL-C,serum SOD,LPL,HL,LA levels of hyperlipidemia rats（P0.01,P0.05）.Shuanghua granules also significantly reduced whole blood viscosity,RV,ηP,IER and IEA（P0.01,P0.05）,and lowered fatty degeneration of liver tissue.Compared with hyperlipidemia model,there was no significant increase in faeces lipoids concentrations.The results confirmed the mechanism of blood lipid regulating effects of Shuanghua granules is probably related with its antioxidation,regulating hemorheology and improving LPA,HL,LA enzymatic activity.

关 键 词：双花颗粒 高脂血症 血脂 脂质过氧化 血液流变学 脂质排泄 

分 类 号：R285.5[医药卫生—中药学]