PAOAA修饰金电极固定过氧化氢酶的电化学研究  

Electrochemical study of Catalase immobilized on PAOOA-modified gold electrode

作　　者：张志军[1] 李曦[1] 肖亮泓[1] 邹传亮[1] 张超灿[2] 

机构地区：[1]武汉理工大学理学院,武汉430070 [2]武汉理工大学材料科学与工程学院,武汉430070

出　　处：《中国科学：化学》2011年第6期1001-1009,共9页SCIENTIA SINICA Chimica

摘　　要：设计了一种基于金属离子螯合法固定蛋白质的新方法.首先电化学聚合苯胺(ANI)/邻氨基苯甲酸(OAA)得到在中性溶液中具有导电性的聚(苯胺-邻氨基苯甲酸)(PAOAA)共聚物膜,并对膜进行了SEM、EDS表征.随后膜负载Cu2+,Cu2+作为螯合离子通过配位作用固定过氧化氢酶(Cat).用EDS测量了铜的负载量为0.49%.脉冲伏安法(DPV)研究了Cat在金电极表面的固定过程,显示随着ANI/OAA在电极表面聚合成膜、Cat在修饰电极表面固定,电极在10mmol/LK3Fe(CN)6/K4Fe(CN)6(Fe(CN)63-/4-)溶液中的扫描峰电流由裸电极的121μA依次降到92.6、71.8μA,表明电极表面修饰的PAOAA膜和固定的Cat阻碍了电荷的传递.电化学阻抗法(EIS)对固定过程中电极表面阻抗变化的研究也得到同样结论.用线性扫描伏安法(LSV)研究了固定到电极表面后Cat对H2O2的催化还原活性.最后以计时电流法(CA)作为检测手段,Cat修饰电极作为探针实现了对H2O2的定量检测,同时该电极显示出良好的重建性.A new method for protein immobilization based on metal-ion chelation was reported in this paper.First,conductivity film poly（aniline-co-2-aminobenzoic acid）（PAOAA）,which is able to conductive in neutral solution,was prepared by electrochemical deposition.The film was characterized by scanning electron microscopy（SEM）and energy-dispersive X-ray（EDS）.Then Cu2＋ions were adsorbed on the film as the chelating ion to immobilize catalase（Cat）.The molar amount of Cu2＋ions in the polymer was 0.49%measured by EDS.Differential pulse voltammetry（DPV）was used to research the immobilization of Cat.The results showed that as PAOAA film forming and Cat immobilizing on the electrode surface,the peak current in 10 mmol/L K3Fe（CN）6/K4Fe（CN）6（Fe（CN）6 3-/4-）decreased from 121μA（bare GE） to 92.6 and 71.8μA respectively.This indicated that PAOAA film and Cat on the electrode could impede the charge transfer.This conclusion is same as the result of electrochemical impedance spectroscopy（EIS）studying in the process of Cat immobilization.The catalytic activity of immobilized Cat for hydrogen peroxide was studied by linear sweep voltammetry（LSV）method.Finally,chronoamperometry（CA）as the detection method,the Cat-modified electrode was able to quantitatively detect hydrogen peroxide.Moreover the Cat-modified electrode could be reuseable.

关 键 词：聚(苯胺-邻氨基苯甲酸) 电化学聚合 蛋白质固定 金属离子螯合 过氧化氢酶 

分 类 号：O646[理学—物理化学]