聚硫堇/过氧化氢光致电化学敏感界面的构建及其对葡萄糖氧化酶催化活性的测定  

Fabrication of poly(thionine)/H_2O_2 photoelectrochemical sensing platform and determination for catalytic activity of glucose oxidase

作　　者：赵常志[1] 俞佳[1] 赵改爽[1] 焦奎[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《中国科学：化学》2011年第6期1075-1080,共6页SCIENTIA SINICA Chimica

基　　金：山东省自然科学基金项目(2009ZRB01393);电分析化学国家重点实验室开放基金的资助

摘　　要：将具有光电活性的硫堇电聚合在光透电极的表面,制备了聚硫堇膜光电极,利用该电极同时具有光敏和电子受体的性能,使其与葡萄糖氧化酶对葡萄糖催化反应产生的电子供体(过氧化氢)发生光致电化学反应,通过检测由此产生的光电流实现对蛋白质催化活性的检测.本文探讨了聚硫堇/过氧化氢光致电化学敏感界面的响应机理,讨论了偏压、电解质溶液pH和底物浓度对光致电化学反应和测定葡萄糖氧化酶催化活性的影响.通过测定商品葡萄糖氧化酶的催化活性,验证了方法的可行性.应用该敏感界面检测蛋白质的催化活性不需要过氧化物酶,比光度法经济、简便和快速.A new photoelectrochemical sensing platform for H2O2 was fabricated by electropolymerization of thionine on ITO electrode.This photosensitive interface was employed as an electron acceptor,and H2O2 proceed from enzymatic catalysis was employed as an electron donor,developing a new determination method for catalytic activity of protein.In the paper,the photoelectrochemical response of poly（thionine）immobilized on ITO electrode to H2O2 was described.The effects of bias voltage,pH of electrolyte and concentration of substrate to the photoelectrochemical response were investigated.The feasibility of method was acknowledged through monitoring the catalytic activity of glucose oxidase.The present method is an economical,speedy,simple and convenient technique to detect the catalytic activity of protein without hydrogen peroxidase.

关 键 词：光致电化学 聚硫堇 过氧化氢 葡萄糖氧化酶 催化活性 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]