人两种胆囊结石的胆汁差异蛋白质组学研究  

Differential proteomics research on gallbladder bile samples from two kinds of cholelithiasis patients

作　　者：谢振玮[1] 王伟[1] 艾开兴[1] 

机构地区：[1]上海交通大学附属第六人民医院普外科,200233

出　　处：《中华实验外科杂志》2011年第6期935-937,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：上海市科委科研基金资助项目（09411964600）;上海市教委科研创新重点项目（09zz22）

摘　　要：目的比较胆固醇性与胆色素性胆囊结石患者的胆囊胆汁蛋白质表达谱差异，寻找两种不同胆囊结石形成及调控相关的蛋白质。方法采集胆囊结石患者胆囊胆汁及结石，结石胆固醇比例〉70％入选胆固醇结石组，胆色素比例〉40％入选胆色素结石组。胆汁予脱盐、脱脂等预处理后，二维色谱／串联质谱分析，Mascot检索数据库。结果分别从胆固醇和胆色素结石胆汁中鉴定出495和511种蛋白质。两组共检测到抗成核因子1种，促成核因子12种，其中Mucin-5B等两种蛋白在胆固醇组高表达，载脂蛋白A-I等11种蛋白在胆色素组高表达。结论二维色谱／串联质谱技术是高效的人胆汁蛋白质组研究技术。两组胆汁的蛋白质差异表达提示两种结石的形成机制差异，胆色素结石形成与促成核因子密切相关，载脂蛋白A—I抗成核作用减弱参与胆固醇结石形成。Objective By comparing gallbladder bile samples from cholesterol cholelithiasis patients with pigment cholelithiasis patients, we try to find out proteins participating in gallstone formation for revealling two kinds of stone formation mechanisms. Methods Gallstones and bile were collected from cholelithiasis patients. These patients were divided into two groups by stone chemical compositon analysis. Patients with stone cholesterol levels greater than 70% were assigned into cholesterol stone group, and those with bilirubin levels greater than 40% into pigment stone group. After removing the bile salt and lipid, peptide fragments were separated by 2D-LC, analysed by MS/MS, and searched against Swiss Prot database by Mascot. Results There were 495 and 511 proteins identified from cholesterol stone group and pigment stone group respectively. There was 1 anti-nucleation factor and 12 pro-nucleation factors, including 2 proteins up-regulated in cholesterol stone group and 11 proteins down-regulated in pigment stone group. Conclusion 2D-LC/MS/MS is an efficient technology in bile proteomics research. Differentially expressed proteins reveal 2 kinds of different stone formation mechanisms. Pro-nucleation factors are closely related with pigment stone formation, while down-regulation of apolipoprotein A- I is closely related with cholesterol stone formation.

关 键 词：胆囊结石 胆汁 蛋白质组学 

分 类 号：R657.4[医药卫生—外科学]