饱和氢盐水对大鼠下肢缺血再灌注及远隔肺组织损伤的保护作用  

Protective effects of hydrogen-rich saline against hind limb ischemia reperfusion injury and associated remote lung injury

作　　者：徐辰[1] 钱汐晶[1] 孙向东[2] 李润平[3] 

机构地区：[1]第二军医大学长海医院,上海200433 [2]第二军医大学长海医院胸心外科,上海200433 [3]第二军医大学潜水医学教研室

出　　处：《中华实验外科杂志》2011年第6期970-972,F0004,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察饱和氢盐水对大鼠下肢组织缺血再灌注及远隔肺组织损伤的保护作用。方法将30只健康sD雄性大鼠随机分为3组：假手术组（S组）、生理盐水组（c组）、氢水治疗组（H组）。建立大鼠下肢缺血再灌注损伤模型（阻断腹主动脉4h，再灌注4h），在再灌注前10min分别注入饱和氢盐水1ml／100g（H组），生理盐水1ml／100g（C组）。再灌注4h后比较肺及腓肠肌湿／干重（W／D）比值、观察病理切片的改变，测定血清、肺及腓肠肌组织中丙二醛（MDA）、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6含量变化。结果H组肌（4．04±0．22）、肺（4．47±0．23）组织湿／干重比低于c组肌（4．67±0．27），肺（4．67±0．27）、组织（P〈0．05），MDA、TNF-α及IL-6血浆[分别为（2．08±1．27）μmol／L、（528．22±221．17）、（41．14±13．01）ng／L]及组织[肺组织分别为（3．58±1．62）μmol／L、（1．17±0．15）、（0．79±0．29）ng／L；肌组织分别为（8．91±3．13）μmol／L、（3．13±0．41）、（3．07±1．69）ng／L）含量均低于C组（P〈0．05），腓肠肌及肺病理学损害明显减轻，与s组比较差异无统计学意义（P〉0．05）。结论缺血再灌注前输注饱和氢盐水可有效保护大鼠下肢缺血再灌注及远隔肺组织的损伤。Objective To explore the protective effects of hydrogen-rich saline against hind limb ischemia reperfusion injury and associated remote lung injury in rats. Methods Thirty Sprague-Dawley rats were randomly divided into three groups： sham group （group S） ; saline control group （group C）, undergoing intravenous infusion of saline （1 mW100 g） at 10 min before reperfusion; hydrogen-treated group （group H）, undergoing intravenous infusion of hydrogen-rich saline （1 ml/100 g） at 10 min before reperfusion. Hind limb ischemia reperfusion model was established by 4 h of the abdominal aorta ligation followed by a 4 h of reperfusion. Rats were killed at the end of reperfusion, and blood samples were collected for determination of serum levels of tumor necrosis factor-α（TNF-α） , interleukin-6 （IL-6） and malonyldialdehyde （MDA）. Specimens of lung and gastrocnemius muscle were collected for measurement of wet-todry weight （W/D） ratio, light microscopic examination and detection of plasma TNF-α, IL-6 and MDA. Results The pathological changes were milder in group H than in group C. The lung （4.47 ± 0. 23 ） and gastrocnemius muscle （4. 04 ± 0. 22） W/D ratio in group H was significantly lower than in group C （4. 67 ±0. 27 vs 4. 67 ±0. 27, P 〈0. 05）. The serum [ （2. 08 ± 1.27） μmol/L, （528. 22 ±221.17）, （41.14 ± 13.01 ） ng/L, respectively ] and homogenate levels [ （3.58 ± 1.62）μmol/L, （ 1.17 ± 0. 15 ）, （0. 79 ±0. 29） ng/L in the lung; （8. 91 ±3.13） μmol/L, （3.13 ±0. 41 ）, （3.07 ± 1.69） ng/L in the gastrocnemius muscle, respectively] of MDA, IL-6 and TNF-α were also significantly lower in group H than in group C （P 〈 0. 05 ）, but there was no significant difference from group S. Conclusion Infusion of hydrogen-rich saline before reperfsion significantly alleviates the hind limb ischemia reperfusion injury and asso-ciated remote lung injury.

关 键 词：饱和氢盐水 肺 骨骼肌 再灌注损伤 自由基 

分 类 号：R285.5[医药卫生—中药学]