HPLC法测定木薯叶中芦丁的含量  

Content Determination of Rutin in Manihot esculenta Leaves by HPLC

作　　者：何翠薇[1] 覃洁萍[1] 黄俏妮[1] 

机构地区：[1]广西中医学院,南宁市530001

出　　处：《中国药房》2011年第23期2160-2161,共2页China Pharmacy

基　　金：广西中医学院自然科学研究课题(P2009030)

摘　　要：目的:建立测定木薯叶中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse XDB C18(0.105006～mm0.×046.06mmgm.,m5Lμ-1m范)围,流内动与相峰为面甲积醇积-分0.1值%呈磷良酸好(1的2:线88性),关流系速(为r=10.m99L.9m9)i;n-平1,均检加测样波回长收为率35为4n1m00。.7结2%果,:R芦SD丁=的2.检3%测(浓n=度6)在。结论:本方法操作简便、准确、重复性好,可作为木薯叶中芦丁的含量测定方法。OBJECTIVE：To establish a method for the content determination of rutin in Manihot esculenta leaves.METHODS：HPLC method was adopted.Agilent ZORBAX Eclipse XDB C18（150 mm×4.6 mm,5 μm） column was used with mobile phase consisted of methanol-0.1% phosphoric acid（12：88） at flow rate of 1 mL·min-1.The detection wavelength was set at 354 nm.RESULTS：The linear range of rutin was 0.006～0.060 mg·mL-1（r=0.999 9） with an average recovery of 100.72%（RSD=2.3%,n=6）.CONCLUSION：The method is simple,accurate,reproducible and could be used for the content determination of rutin in M.esculenta leaves.

关 键 词：高效液相色谱法 木薯叶 芦丁 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]