HPLC-ELSD法测定脑通颗粒中黄芪甲苷和人参皂苷Rb_1的含量及稳定性  

Content Determination of Astragaloside Ⅳ and Ginsenoside Rb_1 in Naotong Granules and Their Stability Observation by HPLC-ELSD

作　　者：陈祖云[1] 石凌云[1] 黄勇[1] 乔希[1] 王爱民[1] 况时详[2] 王永林[1] 

机构地区：[1]贵阳医学院药学院,贵阳市550004 [2]贵阳中医学院附属第二医院,贵阳市550001

出　　处：《中国药房》2011年第23期2164-2166,共3页China Pharmacy

基　　金：贵州省中药现代化科技产业研究开发专项项目(黔科合农字[2006]5023号);贵州省科技计划课题(黔科合计工字[2009]4001号);贵阳市科学技术计划项目([2009]筑科中合同字第9-3-04号)

摘　　要：目的:建立测定脑通颗粒中黄芪甲苷和人参皂苷Rb1含量的方法,并用该方法对制剂中黄芪甲苷和人参皂苷Rb1的稳定性进行考察。方法:采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法。色谱柱为Elipse-XDB-C18(150 mm×4.6 mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1 mL·min-1,柱温为40℃;采用ELSD器,漂移管温度为40℃,雾化器压力为0.30 MPa。结果:黄芪甲苷和人参皂苷Rb1的进样浓度分别在0.058 8～0.940 8 mg·mL-1(r=0.998 9)、0.119 6～1.913 5 mg·mL-1(r=0.999 1)范围内的对数值与各自峰面积的对数值呈良好线性关系;平均加样回收率分别为97.28%、98.53%,RSD分别为3.6%、3.0%(n均为9)。制剂中黄芪甲苷和人参皂苷Rb1含量经加速试验考察,稳定性良好。结论:本方法简便、快速、准确,可用于脑通制剂中黄芪甲苷和人参皂苷Rb的含量测定;经稳定性考察制剂中黄芪甲苷和人参皂苷Rb质量稳定。OBJECTIVE：To establish a method for content determination of astragaloside Ⅳ and ginsenoside Rb1 in Naotong granules,and to study their stability.METHODS：HPLC-ELSD was used.The analysis was carried on Elipse-XDB-C18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of acetonitrile-water（gradient elution） at the flow rate of 1 mL·min-1.The column temperature was 40 ℃.Evaporative light-scattering detector was used.The drift tube temperature was 40℃ and atomizing air pressure was 0.30 Mpa.RESULTS：A linearity was obtained in the range of 0.058 8～0.940 8 mg·mL-1（r=0.998 9） for astragaloside Ⅳ and 0.119 6～1.913 5 mg·mL-1（r=0.999 1） for ginsenoside Rb1 respectively.The average recovery rates of astragaloside Ⅳ and ginsenoside Rb1 were 97.28%（RSD=3.6%,n=9） and 98.53%（RSD=3.0%,n=9）.CONCLUSION：The method is simple,rapid and accurate,and it is suitable for the content determination of astragaloside Ⅳ and ginsenoside Rb1 in Naotong granules.The stability study shows that astragaloside Ⅳ and ginsenoside Rb1 in the granules have stable quality.

关 键 词：高效液相色谱-蒸发光散射检测法 脑通颗粒 黄芪甲苷 人参皂苷RB1 含量测定 稳定性考察 

分 类 号：R283.627[医药卫生—中药学] R927.2[医药卫生—中医学]