绵羊β3肾上腺素能受体蛋白结构和分子进化分析  

Analysis on the Protein Structure and Molecular Evolution of β3-Adrenergic Receptor in Sheep

作　　者：武建亮[1] 乔利英[1] 刘文忠[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国畜牧杂志》2011年第11期15-17,37,共4页Chinese Journal of Animal Science

基　　金：国家自然科学基金(30972084);山西省研究生优秀创新项目(20093011)

摘　　要：β3肾上腺素能受体(ADRB3)是G蛋白耦联受体超家族的成员之一,主要作用于脂肪组织,参与调节脂肪分解和能量代谢。本文基于NCBI上已经发表的14个物种的ADRB3基因mRNA和蛋白质序列,利用网络信息资源和生物学软件,对绵羊等14个物种进行分子进化分析,为进一步研究其表达调控奠定基础。结果表明:该蛋白包含1段50个氨基酸组成的信号肽,是一种具有7个跨膜区结构的不稳定的疏水性膜蛋白。二级结构由α螺旋(23.95%)、β延伸链(20.49%)和无规则卷曲(55.56%)构成;分子进化分析结果显示,14个物种被分成2支,绵羊、山羊、牛、猪、马、狗、猫、豚鼠、大鼠、小鼠、猕猴、黑猩猩和人聚为一支,虹鳟鱼单独为一支,该聚类结果和公认的动物分类及进化关系吻合;非同义替换率与同义替换率的比值ω为3.756,说明ADRB3基因发生了正向选择,即受自然选择的作用较大。The β3-adrenergic receptor（ADRB3） is a G-protein coupled receptor involved in regulating lipolysis and homeostasis.Based on the amino acids（AA） and nucleic acid sequences released by NCBI,the protein structure of ADRB3 in sheep and molecular evolution of its gene in 14 vertebrate animals were analyzed with bioinformatic software and internet resource aiming at analyzing expression and regulation of ADRB3 gene.Results showed that ADRB3 protein contained a 50-AA signal peptide.It was an unstable hydrophobicity membrane protein and had a structure with seven transmembrane domains.The secondary structure was composed of alpha helix（23.95%）,extended strand（20.49%） and random coil（55.56%）.The molecular evolution analysis revealed that the 14 vertebrates were divided into two major branchs,in which,rainbow trout formed one branch alone,and the remaining 13 mammals formed another branch.This phylogenetic tree was consistent well with recognized evolutionary relationship among these species.As revealed by the positive selection results,natural selection may have much influence on ADRB3 gene.

关 键 词：ADRB3基因 蛋白 分子进化 选择 绵羊 

分 类 号：S826.2[农业科学—畜牧学]