利用荷移反应分光光度法测定注射液中的盐酸利多卡因  

Spectrophotometric determination of lidocaine in injections by charge-transfer reaction

作　　者：阿不都克热木.卡地尔 吐尔洪.买买提 买买提明.买合木提 

机构地区：[1]新疆大学化学与化工学院石油天然气精细化工教育部自治区重点实验室,乌鲁木齐830046

出　　处：《分析试验室》2011年第6期19-22,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20765005;21065011);新疆维吾尔自治区高校科研计划青年教师科研启动基金(XJEDU2007S05);新疆大学博士启动基金(BS080113)项目资助

摘　　要：采用分光光度法研究了电子受体2,3-二氯-5,6-二氰基-1,4-对苯醌(DDBQ)与电子供体利多卡因(LD)的荷移反应,发现DDBQ与LD在乙腈中生成稳定的离子缔合物,并通过Job's plot确定DDBQ和LD的摩尔比为1:1。LD与DDBQ在587nm处形成强特征吸收峰。据此建立了一种测定注射液中LD含量的荷移分光光度法。LD浓度在0～8.0×10-4 mol/L范围内符合朗伯一比尔定律,R=0.9984,回收率为102.8%～104.9%,RSD为0.97%～1.2%。离子缔合物的结合常数为5.05×102,自由能变化为-15.45kJ/mol。此方法已用于注射液中LD含量的测定。The charge-transfer reaction of 2, 3-dichloro- 5, 6-dicyano- 1, 4-benzoquinone （DDBQ）as an electron acceptor with lidocaine as an electron donor was investigated by spectrophotometry. In acetonitrile, DDBQ was found to react with lidocaine and produce a stable charge-transfer complex, meanwhile the job＇ s plot has been applied to establish the mole ratio of lidocaine to DDBQ with was found to be 1 ： 1. The addition of lidocaine base to the DDBQ solution cause an immediate change in the absorption spectrum, with new characteristic hand peaking at 587nm. Therefore a highly-selective charge-transfer reaction method for the determination of LD has been developed. The results showed that Beer＇s law is obeyed in the ranges of 0 -8.0 × 10^-4 mot/L for lidoeaine, with R = 0. 9984, the recoveries are from 102. 8% to 104. 9% , and RSDs range from 0.97% to 1.2%. The binding constant of charge-transfer complex is 5.05 × 10^2, and Gibbs free energy is -15.45 k J/tool. The method has been applied to the determination of lidocaine in injections with satisfactory results.

关 键 词：利多卡因 2 5-二氯-3 6-二氰基-1 4-对苯醌 光度法 荷移反应 

分 类 号：O657.3[理学—分析化学]