β-arrestin2对急性支气管哮喘小鼠模型脾CD4＋T细胞T-bet表达的影响  

Effect of β-arrestin 2 on T-bet expression of splenic CD4^＋ T cells in asthmatic murine model

作　　者：王谷宜[1] 刘毅[1] 张莉[1] 刘绍坤[1] 李坑[1] 龚素波[1] 向旭东[1] 陈平[1] 

机构地区：[1]中南大学湘雅二医院呼吸内科,长沙410011

出　　处：《国际呼吸杂志》2011年第11期801-805,共5页International Journal of Respiration

基　　金：湖南省财政厅教育经费专项资助（湘财教指[2008]56号）;湖南省“十一五”重点学科建设经费（湘财教指[2008]69号）

摘　　要：目的探讨G-arrestin 2在急性支气管哮喘（简称哮喘）小鼠模型脾CD4^＋T细胞中的表达及对Tbet表达的影响。方法建立急性哮喘小鼠模型，免疫磁珠分离小鼠脾源性CD4^＋T细胞，RNA干扰β-arrestin 2的表达后，检测CD4^＋T细胞β-arrestin 2、T-bet mRNA和蛋白的表达。结果siRNA-β-arrestin 2-1123沉默效果最佳，相对于未转染siRNA的哮喘组及其他siRNA-β-arrestin2转染组，β-arrestin 2 mRNA明显降低（P〈O．01）；哮喘小鼠模型脾CD4^＋T细胞β-arrestin 2蛋白的表达明显高于正常组（P〈O．01）；β-arrestin 2沉默后，哮喘小鼠脾CD4’T细胞T-bet mRNA和蛋白的表达明显升高（P〈O．05）。结论β-arrestin2可能通过抑制小鼠CD4^＋T细胞T-bet的表达参与哮喘的发病。Objective To detect the expression of β- arrestin 2 in CD4^＋T cells in acute asthmatic murine models as well as the effect of β-arrestin 2 on expression of T-bet. Methods Splenic CD4^＋ T ceils origining from asthmatic murine models were isolated by immunomagnetic bead separation. After β- arrestin 2 expression was knocked down by RNA interferenee,β-arrestin 2 as well as T-bet mRNA and protein expression were determined. Results siRNA Arrb2-1123 had better silencing effect than other siRNA sequences. β-arrestin 2 expression in splenic CD4^＋T cells in asthmatic murine models was significantly higher than that of the normal group （ P 〈0.01）. After β-arrestin 2 expression was knocked down by RNA interference, T-bet mRNA and protein expression in splenic CD4 ^＋T cells in asthmatic mice were significantly increased （P 〈 0.05 ）. Conclusions β-arrestin 2 probably contributes to the pathogenesis of asthma by inhibiting T-bet expression in CD4^＋T cells.

关 键 词：哮喘 CD4^+T细胞 p ARRESTIN 2 T-bet转录因子 

分 类 号：R562.25[医药卫生—呼吸系统]