不同低氧模式对大鼠心肌组织激活蛋白复合物-1的影响  

Effects of different mode of hypoxia on activator protein-1 in cardiac muscle of rats

作　　者：李硕[1] 陈宝元[1] 周伟[1] 万南生[1] 郭润[1] 张祯[1] 

机构地区：[1]天津医科大学总医院呼吸科,天津300052

出　　处：《国际呼吸杂志》2011年第11期850-852,共3页International Journal of Respiration

基　　金：国家自然科学基金资助项目（30770934,30900656）

摘　　要：目的探讨不同低氧模式对大鼠心肌组织氧化还原敏感的转录因子激活蛋白复合物-1（AP1）的影响，以c-fos表达水平间接代表AP-l的激活。方法成年雄性Wistar大鼠96只，随机分为3组：间歇低氧组，持续低氧组和常氧对照组。分别于低氧暴露第2周、第4周、第6周和第8周随机抽取8只大鼠麻醉后处死，留取右心室心肌组织，应用realtimePCR方法检测大鼠心肌组织c-fosmRNA表达水平。结果低氧暴露第6周时，各组间比较c-losmRNA表达水平出现明显差异（F=13．455，P=0．000）。间歇低氧组表达水平明显高于常氧对照组和持续低氧组（F值分别为104．285，45．286，P值分别为0．000，0．037）。继续暴露至第8周，各组间比较差异明显（F=11．279，P=0．000）。间歇低氧组和持续低氧组表达水平均明显高于常氧对照组（F值分别为112，75．375，P值分别为0．00l，0．018）。间歇低氧组和持续低氧组之间差异无统计学意义。结论间歇低氧和持续低氧均可引起大鼠心肌组织cfosmRNA表达的增加。其中间歇低氧可能比持续低氧更容易引起转录因子AP-l的激活，从而介导严重的炎症反应。Objective To investigate the effects of different mode of hypoxia on activator protein-1 （AP-1） in cardiac muscle of rats, which is a transcription factor susceptive to oxidation-reduction. We use the expression of c-fos mRNA to indirectly represents the activation of AP-1. Methods Ninety-six male Wistar rats were divided randomly into the three groups： intermittent hypoxia （IH） group, sustained hypoxia （CH） group and the normal oxygen control （SC） group. At the second, fourth, sixth, and eighth weeks, eight rats under each group were sacrificed to collect cardiac muscles of right ventricle. Real-time PCR assay was used to detect the expression of c fos mRNA. Results After 6 weeks of hypoxic exposure, the expression of c-fos mRNA was found to be significantly different in all three groups （ F =13. 455, P=0. 000）. The expression of c-fos mRNA in IH group was significantly higher than the SC and CH group （ F values were 104. 285 and 45. 286, P values were 0. 000 and 0. 037）. Continued exposure to 8 weeks, there was significant differences among groups （ F =11. 279, P=0. 000）. The expression of c- fos mRNA in IH and CH group were significantly higher than the SC group （ F values were 112 and 75. 375, P values were 0. 001 and 0. 018）. We did not observe the significant differences between the IH and CH groups. Conclusions The increased expression of c-fos mRNA can be caused by intermittent hypoxia and sustained hypoxia. Which intermittent hypoxia may lead to more strongly AP-1 activation than the continuous hypoxia, which induced severe inflammatory reaction.

关 键 词：间歇低氧 阻塞性睡眠呼吸暂停 激活蛋白复合物-1 

分 类 号：R56[医药卫生—呼吸系统]