补体C3基因敲除小鼠的繁育及子代基因型鉴定  

Reproduction and Genotype Identification of Complement 3 Gene Knockout Mice

作　　者：郑静[1] 刘阳[1] 胡松[1] 李敏惠[1] 李丽梅[1] 阳泰[1] 杨淑霞[1] 邹强[1] 

机构地区：[1]成都医学院科研实验中心,成都610083

出　　处：《实验动物科学》2011年第2期7-9,14,共4页Laboratory Animal Science

基　　金：国家自然科学基金(30772771)

摘　　要：目的探讨繁殖和鉴定补体C3基因敲除小鼠的实验方法。方法将所引进的补体C3基因敲除杂合子小鼠(C3+/-)进行饲养并繁殖,其子代出现三种基因型的小鼠,即纯合子C3-/-、杂合子C3+/-、野生型C3+/+,采用ELISA与PCR相结合对子代小鼠基因型进行鉴定。结果繁育出135只子代小鼠,经鉴定,C3+/+、C3+/-、C3-/-各为38只、68只、29只,经χ2检验,子代小鼠分离比例符合孟德尔遗传规律。结论正确的饲养繁殖以及子代鉴定是从杂合子小鼠中获得补体C3基因敲除小鼠的有效途径。Objective This study was to explore the method to breed and identify complement 3 gene knockout mice. Methods The heterozygote of complement 3 gene knockout mice （C3 ＋ / - ） were bred and reproduced. Wild genotype （ C3 ＋ / ＋ ） , heterozygote genotype （ C3 ＋ / - ） , and homozygote genotype （ C3 - / - ） would appear in offspring. The genotypes were characterized using ELISA and PCR. Result The heterozygote mice bred 135 offspring. 38 mice are wild type,68 mice are heterozygote, and 29 mice are homozygote. By x2 text, the segregate ratio of offspring was fitted to the Mendelian ratio. Conclusion Appropriate methods for breeding, reproducing, and identifying are the effective way for acquiring complement 3 gene knockout mice from heterozygote.

关 键 词：补体C3 基因敲除小鼠 PCR ELISA 

分 类 号：Q343.1[生物学—遗传学]