封闭群长爪沙鼠遗传标准的建立  

Establishment of Genetic Standards of Closed Colony Gerbil

作　　者：李薇[1] 江其辉[2] 杜小燕[1] 尚世臣[2] 陈振文[1] 

机构地区：[1]首都医科大学基础医学院实验动物学系,北京100069 [2]军事医学科学院实验动物中心,北京100071

出　　处：《实验动物科学》2011年第2期31-36,共6页Laboratory Animal Science

基　　金：国家科技支撑计划课题(No:2009BAI83B02-41);北京市教委重点课题(KZ200910025002)

摘　　要：目的建立封闭群长爪沙鼠遗传质量标准和遗传质量检测方法。方法根据群体遗传学理论,参照实验动物哺乳类实验动物的遗传质量控制(GB14923-2001),在查阅大量文献资料的基础上,建立封闭群长爪沙鼠遗传标准。检测方法采用微卫星标记分析技术。从GeneBank中挑选出的536个小鼠微卫星位点对长爪沙鼠基因组DNA进行PCR扩增得到135个长爪沙鼠微卫星,并优化PCR扩增条件,通过琼脂糖电泳和STR扫描二级筛选,根据位点在染色体分布情况、等位基因数目和多态性等优化出适于封闭群长爪沙鼠遗传检测的微卫星位点组合,建立检测方法。结果初步建立了封闭群长爪沙鼠遗传质量标准和检测方法。Objective To establish genetic quality standards of closed colony gerbil and its detecting methods. Methods Based on investigating a large amount of documents and data,genetic standards of closed colony gerbil were established according to laboratory animal-Genetic quality control of mammalian laboratory animals GB 14923- 2001 and theory of population genetics. The method was established on microsatellite markers, which is selected from 536 mouse microsatellite markers chosen from Gene Bank to identify polymorphic dinucleotide repeat loci in the gerbil by cross-ampli-cation, and then by agarose gel electrophoresis assay and STR loci gene scan analysis. Finally, microsatellite loci in closed colony gerbil were selected in the light of allele number and polymorphism, and detecting methods were established. Conclusion To some extent, Genetic standards of closed colony experimental gerbil o and its detecting methods have been established.

关 键 词：长爪沙鼠 微卫星位点 遗传标准 

分 类 号：R341[医药卫生—基础医学]