益气活血化痰法对酒精性肝纤维化大鼠转化生长因子-β1/Smads信号通路的影响  

Effect of Yiqi Huoxue Huatan Method on the Transformation Growth Factor-β1/Smads Signal Pathway of Rats with Alcoholic Liver Fibrosis

作　　者：吴凌康[1] 史亮亮[1] 陈春梅[1] 单国栋[2] 吴峰[2] 

机构地区：[1]浙江中医药大学附属第二医院,浙江省杭州市潮王路318号310005 [2]浙江大学医学院附属第一医院

出　　处：《中医杂志》2011年第12期1046-1049,共4页Journal of Traditional Chinese Medicine

摘　　要：目的探讨益气活血化痰法对大鼠酒精性肝纤维化的影响及机制。方法将120只SD大鼠随机分成对照组、模型组、秋水仙碱组、小剂量中药组、中剂量中药组、大剂量中药组各20只。除对照组外,其余各组大鼠均用56°酒精灌胃[15ml/(kg.d)-1]致肝纤维化。同时,各小、中、大剂量中药组分别以10g/(kg.d)-1、20g/(kg.d)-1、40g/(kg.d)-1益气活血化痰法中药组方灌胃,秋水仙碱组以秋水仙碱灌胃[2mg/(kg.d)-1],模型组予等量生理盐水灌胃。6个月后处死取肝,采用HE染色考察各组大鼠肝脏的纤维化程度,免疫组化法检测肝组织转化生长因子-β1(TGF-β1)蛋白表达,用RT-PCR法检测各组大鼠肝组织中TGFβ-1和Smads3、Smads4、Smads7的核酸水平表达。结果不同剂量中药组及秋水仙碱组的纤维化分期均与模型组比较差异有统计学意义(P<0.05或P<0.01);与模型组比较,各中药组及秋水仙碱组的TGFβ-1蛋白表达均显著降低(P<0.01),且大剂量中药组较秋水仙碱组TGFβ-1蛋白显著降低(P<0.01);不同剂量与治疗后TGF-β1、Smad3、Smad4、Smad7各核酸分子的表达水平均有相关性。结论益气活血化痰法能下调酒精性肝纤维化大鼠TGFβ-1、Smad3、Smad4 mRNA表达水平并上调smad7 mRNA表达水平,干预了Smads信号通路的表达。Objective To explore the effect and mechanism of Yiqi Huoxue Huatan Method（treatment of reinforcing qi,activating blood,and dissolving phlegm） on alcoholic liver fibrosis rats.Methods Totally 120 SD rats were randomized into control group,model group,colchicin group,and small-dosage,medium-dosage,and large-dosage Chinese medicine groups,with 20 in each.Except the control group,the other groups were all treated with alcohol intragastric feeding [15ml/（kg·d）-1] to induce liver fibrosis.Meanwhile,the small-dosage,medium-dosage,and large-dosage Chinese medicine groups were given intragastric feeding with herbal decoction made by Yiqi Huoxue Huatan medicines,10g/（kg·d）-1,20g/（kg·d）-1,and 40g/（kg·d）-1 respectively.The colchicin group was administered colchicin [2mg/（kg·d）-1] by intragastric feeding.And the model group was applied equal amount of normal saline for intragastric feeding.Six months later,the rats were killed and their livers were obtained for observation.The severity of fibrosis was determined by using the HE dyeing.The transformation growth factor-β1（TGF-β1） expression and distribution in the liver tissues of all groups were detected with the immunohistochemical method.And the expression of TGF-β1 and Smads3,Smads4,and Smads7 nucleic acid in liver tissues of all groups was tested with RT-PCR method.Results The stage of fibrosis in the different dosage Chinese medicine groups and colchicin group was all different from that of the model group with statistical significance（P〈0.05 or P〈0.01）.Compared with the model group,the expression of TGF-β1 in all Chinese medicine groups and colchicin group was significantly decreased（P〈0.01）,and the decrease in the large-dosage Chinese medicine group was more significant than that of the colchicin group（P〈0.01）.The dosage of Chinese medicine was correlated with the expression level of TGF-β1,Smad3,Smad4,and Smad7 nucleic acid molecules after treatment.Conclusion Yiqi Huoxue Huatan method is effective to down-regu

关 键 词：酒精性肝纤维化 益气活血化痰 转化生长因子-Β1 SMADS信号通路 

分 类 号：R285.5[医药卫生—中药学]