培养的牛心内膜内皮细胞水解细胞外腺苷酸<英文>  

Hydrolysis of extracellular adenine nucleotides by cultured bovine endocardial endothelial cells

作　　者：易富贤[1] 刘文兰[1] 谌立伟[1] 曾珊[1] 郭兆贵[1] 

机构地区：[1]湖南医科大学分子药理研究室,长沙中国410078

出　　处：《中国药理学报》1999年第8期745-749,共5页Acta Pharmacologica Sinica

摘　　要：目的:研究牛心内膜内皮细胞(BEEC)腺苷三磷酸双磷酸酶(apyrase)的特性并比较牛心内膜及血管内皮细胞的胞外腺苷酸酶活性。方法:以反相HPLC测定核苷酸含量,以磷离子释放法检测apyrase活性。结果:叠氮钠10mmol·L^(-1)和氟化钠20mmol·L^(-1)分别抑制BEEC apyrase 51％及38％活性,而Na^+/K^+ -ATP酶抑制剂哇巴因则对BEEC apyrase活性无影响。过氧化氢0.5mmol·L^(-1)呈时间依赖性地抑制心内膜内皮细胞apyrase活性。BEEC的apyrase活性低于主动脉内皮细胞的apyrase活性,而BEEC的胞外AMP酶则远较血管内皮细胞的活性高。结论:BEEC apyrase具有叠氮钠和氟化钠敏感的、哇巴因不敏感的特性;与血管内皮细胞相比,BEEC apyrase活性较高而胞外AMP酶活性较低。AIM: To characterize the ATP diphosphohydrolase (apyrase) of bovine endocardial endothelial cells, and to compare ecto-adeninenucleotidase activity between bovine endocardial and aortic endothelial cells (BEEC and BAEC). METHODS: The nucleotide was analyzed by reversed phase HPLC and apyrase activity was assayed by inorganic phosphate release. RESULTS: Apyrase inhibitors, both NaN3 10 mmol·L-1 and NaF 20 mmol·L-1, inhibited BEEC apyrase activity by 51 % and 38 %, respectively. The inhibitor for Na+/K+-ATPase, ouabain, did not affect the enzyme activity. Edetic acid 5 mmol·L-1 completely inhibited the enzyme activity. H2O2 0.5 mmol·L-1 downregulated BEEC apyrase activity in a time-dependent manner. The apyrases activities in BAEC were higher than those in BEEC, while the ecto-AMPase activity in BAEC was much weaker than that in BEEC. CONCLUSION: BEEC have NaN3- and NaF-sensitive, ouabain-insensitive apyrase activity. BEEC had high ecto-AMPase activities, and low apyrases activities as compared with BAEC.

关 键 词：心内膜 血管内 腺苷酸类 叠氮钠 APYRASE 

分 类 号：Q463[生物学—生理学] R331.32[医药卫生—人体生理学]