苦豆子根瘤内生细菌分离及其对棉花枯萎病的生物防治效果测定  

Isolation of endophytic bacteria from nodule of Sophora alopecuroides and effect of biological control against Fusarium wilt

作　　者：龚明福[1,2] 韩松[2] 李超[2] 徐琳[1,3] 韦革宏[1] 

机构地区：[1]西北农林科技大学生命科学学院陕西省农业分子生物学重点实验室,陕西杨陵712100 [2]塔里木大学生命科学学院新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [3]河西学院生命科学与工程系,甘肃张掖734000

出　　处：《微生物学通报》2011年第6期865-870,共6页Microbiology China

基　　金：国家自然科学基金项目(No.30960010;30670372);科技部农业微生物菌种资源整理;整合及共享试点子项目(No.2005DKA21201)

摘　　要：对从塔里木盆地苦豆子中分离得到的内生细菌进行皿内涂布拮抗实验、对峙培养法拮抗实验、胞外分泌物拮抗性测定和盆栽控病实验等研究,结果表明塔里木盆地苦豆子根瘤中存在大量的棉花枯萎病菌拮抗性内生细菌。皿内涂布法筛选结果表明60株苦豆子根瘤内生细菌中有48株相对抑菌率超过50%。对峙培养法对48株拮抗性内生细菌进一步筛选的结果表明,对棉花枯萎病菌抑菌距离超过20 mm的菌株有40株。40株拮抗性内生细菌胞外分泌物对棉花枯萎病菌的抑菌距离超过5 mm的有26株。KDRE12和KDRE41对棉花枯萎病的盆栽控病平均防效分别为67.11%和72.65%,具有应用潜力。Lots of endophytic bacteria strains isolated from healthy Sophora alopecuroides were studied by using surface spread plate experiment,confront antibiotic culture experiment,antagonistic activities of extracellular secretions determination and disease-control experiment in greenhouse.The results in-dicated that there were a lot of endophytic bacteria resources isolated from nodule of Sophora alopecuroides antagonized against Fusarium oxysporum.48 strains with the relative inhibition rate more than 50% of total 60 endophytic bacteria were selected with surface spread plate method.48 an-tagonistic strains were then screened in confront antibiotic culture trial.The results showed that 40 strains had inhibits against Fusarium oxysporum with more than 20 mm inhibition zone,and 26 strains with more than 5 mm in extracellular secretions inhibitory activities testing experiment on 40 antago-nistic strains.In disease-control experiments with Fusarium oxysporum carried out in greenhouses,two isolates（KDRE12 and KDRE41） gave satisfactory results,with 67.11% and 72.65% average control effect,respectively,which have good applied potential.

关 键 词：苦豆子 根瘤内生细菌 棉花枯萎病 生物防治 拮抗 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治] Q93[农业科学—植物保护]