HBxAg反式调节基因11剪切体的克隆化及生物信息学分析  

Cloning and bioinformatics analysis of gene 11 spliced variant transregulated by hepatits B virus X protein

作　　者：尹丽[1,2] 林原[1] 唐泽耀[1] 成军[3] 

机构地区：[1]大连医科大学药学院药理学教研室,辽宁大连116044 [2]新疆维吾尔自治区食品药品检验所,新疆乌鲁木齐830003 [3]北京地坛医院传染病研究所,北京100015

出　　处：《中国生化药物杂志》2011年第3期183-186,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：北京市科委重大科技项目(NO.D08050700650803);国家重点基础研究973项目NO.2004(B518908)

摘　　要：目的克隆乙型肝炎病毒(HBV)X蛋白反式调节的靶基因11(XTP11),获得未知功能的新基因-XTP11剪切体,对其进行生物信息学分析,探讨其结构和功能。方法从肝母细胞瘤细胞系HepG2中提取总RNA,逆转录为cDNA后,设计特异性引物克隆XTP11剪切体,生物信息学技术获得编码序列、物理化学性质、蛋白质结构和功能等基本信息。结果成功扩增出XTP11剪切体基因,生物信息学分析推定ORF为1 314个核苷酸,编码产物为437个氨基酸残基。结论此文发现并鉴定了HBV XTP11剪切体,为阐明其在HBV X蛋白致病机制中的作用提供新的研究线索。Purpose To clone a new gene 11 spliced variant transregulated by hepatitis B virus X protein,and to explore its structure and function by bioinformatical analysis.Methods The total RNA was extracted from hepatoblastoma cells HepG2.XTP11 spliced variant was amplified from reverse transcribed cDNA by polymerase chain reaction using specific primer.Bioinformatic methods were used to analyze the coding sequence,physico-chemical propsrties,structure and function of XTP11 spliced variant.Results Spliced form of XTP11 was amplified successfully by RT-PCR from HepG2 cDNA.Bioinformatical analysis showed that its new open reading frame（ORF） is 1 314 bp in length and translated a protein containing 437 amino acid residues.Conclusion XTP11 spliced variant has been analyzed using bioinformatics,which provides some clues for studying the pathogenesis of XTP11 spliced variant in the occurrence of HBV.

关 键 词：乙型肝炎病毒 剪切体 克隆化 生物信息学 

分 类 号：R512.62[医药卫生—内科学] Q78[医药卫生—临床医学]