椿根皮中生物碱及苦木素类化合物的分离与鉴定  

Isolation and structure identification of alkaloid and quassinoids from root bark of Ailanthus altissima

作　　者：齐鑫[1] 陈智华[1] 高璐[1] 徐丽丽[1] 于波[1] 杨红[1] 

机构地区：[1]辽宁师范大学生命科学学院辽宁省生物技术与分子药物研发重点实验室,辽宁大连116029

出　　处：《中草药》2011年第6期1057-1060,共4页Chinese Traditional and Herbal Drugs

基　　金：大连市科技局科技计划项目(2008E11SF162)

摘　　要：目的利用色谱法分离与鉴定椿根皮Ailanthus altissima的化学成分。方法采用乙醇提取法对椿根皮进行回流提取,应用制备液相色谱法对椿根皮粗提物进行分离纯化,分离化合物的结构由质谱和核磁共振氢谱、碳谱鉴定。结果分离纯化得到臭椿苦酮(1)、臭椿辛内酯A(2)和一新化合物铁屎米酮糖酯(3),且质量分数均在95%以上,化合物3为一个新的β-卡波啉生物碱。结论该法可以快速、准确地对椿根皮中含量较低的化合物进行提取与鉴定。Objective Isolation and identification of chemical components from the root bark of Ailanthus alt＆sima by HPLC method. Methods Crude products which were extracted with ethanol were separated and purified by preparative HPLC, and the structures of the compounds were identified by mass spectrometry （MS）, 1H-NMR, and 13C-NMR. Results Under the above conditions, ailanthone （1）, shinjulactone A （2）, and a new β-carboline alkaloid （3） were successfully obtained, and the purities of the compounds are all above 95%. Conclusion A rapid and simple method is set up to isolate and identify lower content components from A. altissima.

关 键 词：椿根皮 苦木素 β-卡波啉生物碱 铁屎米酮糖酯 高效液相制备色谱 

分 类 号：R284.1[医药卫生—中药学]