HPLC测定不同来源大黄中蒽醌和二蒽酮类成分  

Determination of anthraquinones and bianthrones in rhubarb from different sources by HPLC

作　　者：王哲[1] 许利嘉[1] 何春年[1] 彭勇[1] 肖培根[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中草药》2011年第6期1114-1118,共5页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础发展研究计划(2006CB504701);国家科技重大专项(2008ZX10005-004);国家自然科学基金重点项目(30530860)

摘　　要：目的建立同时测定大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和番泻苷A、番泻苷B的HPLC法,并对不同来源的大黄样品进行测定。方法采用HPLC梯度洗脱,色谱柱为Agilent Eclipse XDB-C18(150 mm×4.6 mm,5μm);检测波长分别为254 nm、340 nm;体积流量为1 mL/min;柱温为30℃。结果在一定范围内,芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和番泻苷A、番泻苷B峰面积积分值与进样量线性关系良好,加样回收率符合要求,方法具有较好的精密度、重现性和稳定性,可同时对多种来源大黄药材进行测定。结论不同来源大黄的化学成分差异较大,其中种植大黄样品中总蒽醌及总番泻苷的量均少于野生样品。Objective To develop a HPLC method for determining the content of aloe-emodin,rhein,emodin,chrysophanol,physcion,sennoside A,and sennoside B simultaneously.The method was used in content determination of constituents in rhubarb from different sources.Methods The separations were carried out at 30 ℃ on an Agilent Eclipse XDB-C18 column（150 mm × 4.6 mm,5μm） and eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode.The flow rate was 1.0 mL/min,detection wavelengths were 254 nm for aloe-emodin,rhein,emodin,chrysophanol,physcion and 340 nm for sennoside A and sennoside B,column temperature was 30 ℃.Results The peak areas and injection ammounts of aloe-emodin,rhein,emodin,chrysophanol,physcion,sennoside A and sennoside B showed a good linear relationship within a certain range.The average recoveries were standards-compliant.The method is simple,accurate and repeatable to be used in content determination of rhubarb.Conclusion The total anthraquinones content and total sennosides content in cultivated samples are much less than those in the wild samples.

关 键 词：大黄 葸醌 番泻苷 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 

分 类 号：R286.02[医药卫生—中药学]