微通道内高通量磁泳分离实验研究  

An experimental study on magnetophoretic separation with high-throughput applications in a microchannel

作　　者：吴信宇[1] 吴慧英[1] 唐慧敏[1] 

机构地区：[1]上海交通大学机械与动力工程学院,上海200240

出　　处：《功能材料》2011年第6期1147-1150,共4页Journal of Functional Materials

基　　金：国家杰出青年科学基金资助项目(50925624);上海市曙光计划跟踪资助项目(08GG05)

摘　　要：磁泳是实现生物分离的主要手段之一。利用功能磁珠在微流控芯片上实现高效磁泳分离是近年来的研究热点。对直径为1μm的超顺磁磁珠在当量直径为114.3μm的矩形微通道内的磁泳分离特性进行了实验研究。利用高速CCD观测了磁珠在微通道内捕获与释放的全过程,并通过图像分析,得到了磁珠的捕获情况随时间及流速的变化规律。实验发现,在高流速时,捕获数量随着时间呈现线性增加,而在低流速时,捕获数量增加的速度随时间逐渐变缓;在一定磁场下,存在一个临界流体速度,当流体速度小于此临界速度,捕获磁珠数较多,大于此临界速度,捕获数量则迅速下降,且下降速度随流速增加而趋缓。研究对认识微通道内磁珠运动规律以实现快速高效的磁泳分离具有一定的指导意义。Magnetophoresis is one of the most popular approaches for biological separations.Magnetophoresis on microfluidic chips by utilizing functional magnetic beads has become a hot topic of researc h in recent years.In this paper,an experimental study on magnetophoretic separation characteristics was carried out by using superparamagnetic beads with the diameter of 1μm in a rectangular microchannel having hydraulic diameter of 114.3μm.The capture and release processes of the magnetic beads in the microchannel were observed and recorded with the help of high speed CCD camera.The relations between the number of captured beads and time and fluid velocity were obtained by imaging analysis.The results show that the number of captured magnetic beads increases linearly with time at high flow rates while at low flow rates it increases initially quickly and then slowly with time.It is also found that for magnetophoretic separation in microchannels there exists a critical flow rate above which the number of captured beads will drop rapidly to a relatively small value and then decreases slowly when the flow rate continues to increase.It is anticipated that the results in this paper are helpful for the optimum design of highly efficient magnetophoretic separators.

关 键 词：微流 功能磁珠 磁泳分离 高通量 微通道 

分 类 号：O658[理学—分析化学]