葛根素对脑死亡大鼠肝脏的保护作用  

Hepatoprotective effects of puerarin on brain death rats

作　　者：方达明[1] 朱毅[1] 眀英姿 牛英[1] 刘炼[1] 叶啟发[1] 

机构地区：[1]中南大学湘雅三医院卫生部移植医学工程技术研究中心,湖南长沙410013

出　　处：《中国普通外科杂志》2011年第5期503-506,共4页China Journal of General Surgery

基　　金：中央高校基本科研业务费专项基金(303275111,303275899)

摘　　要：目的探讨葛根素对脑死亡状态下大鼠的肝脏的保护作用。方法 SD大鼠随机分为3组:模型组,采用渐进式颅内加压法建大鼠脑死亡模型;葛根素组,建脑死亡模型后给予葛根素;假手术组,仅做假手术,不建脑死亡模型。分别于造模后2 h和4 h取材,检测血清中ALT,AST,TNF-α和IL-6水平,并做肝脏病理形态学观察。结果与假手术组比较,模型组与葛根素组大鼠血清ALT,AST,TNF-α和IL-6水平均明显升高(均P<0.05),同组不同时间点比较显示,模型组与葛根素组大鼠上述指标且均呈进行性升高(均P<0.05),在相同时间点上,葛根素组大鼠上述指标明显低于模型组大鼠(P<0.05);病理学检查显示,模型组与葛根素组肝脏均出现不同程度的损伤,但葛根素组损伤程度明显轻于模型组。结论葛根素对脑死亡状态下大鼠肝脏具有作用。Objective To test the hepatoprotective effects of puerarin on brain death rats.Methods The male SD rats were randomly divided into 3 groups： brain death group（brain death models were established using progressive intracranial compression methods）,puerarin treated group（rats were treated with puerarin after brain death was established） and sham-operated group（rats underwent sham operation without brain death insult）.Blood and liver samples of the rats were harvested at 2 h and 4 h after brain death to measure the serum levels of ALT,AST,TNF-α and IL-6,and to examine morphological changes of liver,respectively.Results The serum levels of ALT,AST,TNF-α and IL-6 were significantly increased in both brain death group and puerarin treated group compared with sham-operated group（both P0.05）.The above parameters showed progressive increase in both brain death group and puerarin treated group compared with themselves at the different time points（both P0.05）.However,the above parameters in puerarin treated group were significantly lower than those in brain death group at the same time point（P0.05）.The pathological examination demonstrated that the liver tissues were damaged to different extents in both brain death group and puerarin treated group,but the injuries in puerarin treated group were remarkably milder than those in brain death group.Conclusions Puerarin has hepatoprotective effects on rats after brain death.

关 键 词：肝/代谢 脑死亡 葛根素 大鼠 

分 类 号：R333.4[医药卫生—人体生理学]