肝硬化疾病评分模型实验研究  

Experimental study of scoring model for liver cirrhosis diseases

作　　者：王正华[1] 郑军[1] 王群兴[2] 罗春华[2] 王雅琴[3] 

机构地区：[1]三峡大学第一临床医学院普通外科,宜昌443003 [2]三峡大学第一临床医学院检验科,宜昌443003 [3]三峡大学医学院病理研究室,宜昌443002

出　　处：《第二军医大学学报》2011年第5期485-489,共5页Academic Journal of Second Military Medical University

基　　金：三峡大学培优基金(2010PY061)~~

摘　　要：目的建立实验性大鼠肝硬化疾病评分模型(scoring model for liver cirrhosis disease,SLCD),为临床早期预防及治疗肝硬化疾病提供参考依据。方法采用复合因素法建立实验性大鼠肝硬化诱导模型,对肝硬化诱导过程中不同时相点大鼠肝组织损害程度和血清学肝功能储备变化进行动态观察。运用物元分析法获得实验性大鼠SLCD评分公式。结果实验性大鼠肝硬化模型复制成功。选取年龄、血清总胆红素(TBil)、血清白蛋白(Alb)、血清前白蛋白(PA)、凝血酶原时间国际标准化比值(PT-INR)、血清肌酐(SCr)等6个检测指标,成功构建了实验性大鼠SLCD及SLCD评分公式。评估程度以R值表示,随着病变程度和纤维化程度加重,R值逐渐降低。R=1表示肝脏组织正常;0.702≤R<1表示肝脏炎症反应和局灶性变性、坏死;0.542≤R<0.702表示肝脏出现纤维组织增生现象;0.352≤R<0.542有假小叶形成,为肝硬化期;R<0.352表示肝硬化后期。结论 SLCD评分公式能较敏感而准确地反映实验性肝硬化大鼠肝脏损害及肝功能储备情况,若能在临床进一步证实其运用价值,则可为临床早期预防及治疗肝硬化疾病提供参考依据。Objective To establish a scoring model for liver cirrhosis disease（SLCD） in experimental rats,so as to provide evidence for early clinical prevention and treatment of liver cirrhosis diseases.Methods The liver cirrhosis model was induced in rats by composite factor method.The damages of hepatic tissues and the changes of serological liver function were observed in different phases of the rat liver cirrhosis model.The SLCD formula was obtained using the Matter-Element Analysis method.Results The experimental liver cirrhosis model was successfully established in rats.The experimental rat SLCD and SLCD formula were successfully constructed using the following 6 parameters,the age,total bilirubin,albumin,prealbumin,prothrombin time-international normalized ratio,and serum creatinine.The score was expressed as the R value,which gradually decreased with the aggravation of lesion and fibrosis.R=1： the liver tissue was normal;0.702≤R1： there were inflammatory reaction,focal degeneration and necrosis in the liver;0.542≤R0.702： there was fibroplasia in the liver;0.352≤R0.542： pseudolobules were formed,and it was in the liver cirrhosis stage;and R0.352： it was in the later stage of liver cirrhosis.Conclusion The SLCD model can sensitively and accurately display the liver impairment and liver function reserve during experimental liver cirrhosis in rats.It may help the early prevention,diagnosis and treatment of the cirrhosis diseases if relevant clinical evidence is obtained.

关 键 词：实验性肝硬化 评分模型 物元分析法 

分 类 号：R657.31[医药卫生—外科学]