植物生理学实验中一类偶氮化合物的分光光度检测  

Detection of a Kind of Azo Compound in Plant Physiology Experiment by Spectrophotometer

作　　者：刘少霞[1] 陈贵[1] 秦萍[1] 姚远[2] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110866 [2]沈阳农业大学科技开发公司,沈阳110866

出　　处：《植物生理学报》2011年第5期521-524,共4页Plant Physiology Journal

摘　　要：偶氮化合物在可见光特定波长下有最大吸收峰,但对这一特定波长认定目前仍不统一。本试验利用分光光度计法测定了偶氮化合物的特定吸收峰。结果表明:NO2-、对氨基苯磺酸(或磺胺)及α-萘胺在酸性条件下形成偶氮化合物特定吸收峰在A520nm处,并非A510nm、A530nm或A540nm。It has been known that the absorption peak of azo compound will be observed under specified wavelength of visible light.However,the specified wavelength is inconsistent in different references to data.The visible absorption peak of azo compound was determined by spectrophotometry in this paper.The azo compound was generated by using the combination of naphthylamine with aminobenzene sulfonic acid,or sulfanilamide.The result shows that the absorption peak of azo compound is in A520 nm,rather than in A510 nm,A530 nm or A540 nm which are often used in the references.

关 键 词：偶氮化合物 吸光度 磺胺 对氨基苯磺酸 Α-萘胺 

分 类 号：Q945[生物学—植物学]