顺铂诱导化疗损伤性卵巢早衰大鼠模型的探讨  

Investigate an Method of Cisplatin Inducement in Establishment of the Rats Model for Chemotherapy Induced Premature Ovarian Failure

作　　者：任莉[1] 徐琳[2] 韩雪松[2] 普苹[3] 刘攀 

机构地区：[1]云南省第一人民医院妇科,昆明650031 [2]昆明医学院第一附属医院妇产科,昆明650031 [3]昆明医学院第一附属医院病理科,昆明650032 [4]昆明市延安医院呈贡新区医院外科,昆明650500

出　　处：《生殖与避孕》2011年第5期294-298,共5页Reproduction and Contraception

摘　　要：目的:探讨顺铂诱导大鼠化疗损伤性卵巢功能早衰大鼠模型的可行性。方法:成熟雌性SD大鼠腹腔注射低、高剂量顺铂4.5 mg/kg(A组)、6.0 mg/kg(B组)和生理盐水(C组),每周1次,共2次,建立大鼠化疗损伤性卵巢早衰模型。检测血清FSH水平及光学显微镜下计数卵巢最大切面原始卵泡、初级卵泡、闭锁卵泡,阴道涂片观察动情周期变化。结果:A、B组大鼠动情周期均明显长于C组(P<0.05),并呈现剂量相关性改变。B组动情周期天数明显长于注射前(P<0.01);血清FSH水平A组与C组相比无统计学意义(P>0.05),B组明显高于A组和C组(P<0.05)。各顺铂组血清FSH水平注射后均较注射前明显升高(P<0.05)。腹腔注射顺铂后,A组、B组大鼠卵巢最大切面原始卵泡数和初级卵泡数均明显降低(P>0.05),而闭锁卵泡数均明显增加(P<0.05),并呈现剂量相关性改变。结论:顺铂可诱导化疗损伤性卵巢早衰。此化疗损伤性卵巢早衰大鼠模型血FSH明显升高,卵巢组织学衰退性改变与人类化疗损伤性卵巢早衰病变过程相似。Objective： To investigate an applicable method of cisplatin inducement in establishment of the rats model for chemotherapy induced premature ovarian failure（POF）.Methods： Adult female SD rats were divided into 3 groups： by treated with the low dose of cisplatin 4.5 mg/kg in gruop A,the high dose of cisplatin 6.0 mg/kg in group B and with saline in group C（the control）,once a week for 2 weeks.Rats model of chemotherapy induced POF was establish successfully by intraperitoneal injection.The serum FSH level was measured by ELASA method and the number of original,primary and atresic follicles was counted by using paraffin sections under optical microscope in the largest ovarian cross-section.Vaginal smear record changes in the estrous cycle.Results： Duration of estrous cycle with different doses of cisplatin in groups A and B,as significantly longer than that of group C（P0.05）.And it took on a dose-related change.Estrous cycle of group B was obviously longer after intraperitoneal injection than before the injection（P0.01）.For the serum FSH levels,there was no statistical significance between group A and group C（P0.05）,but significantly higher in group B（P0.05）.The serum FSH level as significantly higher in both cisplatin groups after injection than before the injection（P0.01）.The number of original and primary follicles was significantly reduced in group A and B（P0.05）,but the number of atretic follicles was significantly increased in a dose-related change after injection（P0.05）.Conclusion： Cisplatin can lead to chemotherapy induced POF in rats.The reproductive endocrinology and pathological changes in this model are similar with that of chemotherapy induced POF in human.

关 键 词：卵巢早衰(POF) 顺铂 化疗 大鼠 动物模型 

分 类 号：R711.75[医药卫生—妇产科学]