磷脂酰肌醇3-激酶／蛋白激酶B信号转导通路在苯醌致HL-60细胞增殖过程中的作用  

Effect of phosphatidylinositol 3-kinase/serine-threonine kinase signalling pathway during the proliferation process of HL-60 cells exposed to benzoquinone

作　　者：刘移民[1,2] 李永胜[3] 王致[1] 李旭东[4] 郭晓 顾春晖[2] 

机构地区：[1]广州市职业病防治院,510620 [2]中山大学公共卫生学院 [3]广东省东莞市疾病预防控制中心 [4]广东省职业病防治院 [5]广州市卫生监督所

出　　处：《中华劳动卫生职业病杂志》2011年第5期334-337,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广州市科技攻关项目（200523.E0131）;广东省医学科研基金项目（B2009218）

摘　　要：目的探讨磷脂酰肌醇3-激酶／蛋白激酶B（P13K／Akt）信号转导通路在苯醌（BQ）致HL-60细胞增殖中的作用。方法取对数生长期的HL-60细胞，分为对照组（PBS处理细胞）、BQ染毒组（3μmol／LBQ染毒）、LY294002＋BQ染毒组（3μmol／LBQ染毒前加20μmol／LLY294002），用alamar blue法检测细胞增殖率，免疫印迹（Western blot）法检测细胞内p-Akt、Akt蛋白的表达，流式细胞仪检测细胞周期情况。结果BO染毒组细胞增殖率（185．00％±30．00％）和S、G2期细胞比例（分别为48．23％±1．37％、15．40％±1．21％）均高于对照组（分别为100．00％±0．00％、42．47％±0．45％、5．40％±0．40％），G1期细胞比例（36．37％±0．40％）低于对照组（52．13％±0．75％），差异均有统计学意义（P〈0．05）；BQ染毒组细胞内p-Akt蛋白表达量明显高于对照组，差异有统计学意义（P〈0．05），Akt表达量与对照组的差异无统计学意义（P〉0．05）。LY294002＋BQ染毒组细胞增殖率（82．59％±15．00％）和S、G2期细胞比例（分别为42．03％±0．50％、3．87％±0．47％）比BQ染毒组低，G1期细胞比例（54．43％±0．40％）比BQ染毒组高，差异均有统计学意义（P〈0．05），细胞内p-Akt蛋白表达量明显低于BQ染毒组，差异有统计学意义（P〈0．05）；Akt蛋白表达量与BQ染毒组的差异无统计学意义（P〉0．05）。结论P13K／Akt信号转导通路在BQ致HL-60细胞增殖过程中可能起着重要作用。Objective To explore the effects of phosphatidylinositol 3-kinase/Serine-threonine kinase （PI3K/Akt） signal pathway on the proliferation of HL-60 cells exposed to benzoquinone （BQ）. Methods HL- 60 cells were divided into 3 groups： control group （treated with PBS）, BQ group （treated with 3 μmol/L BQ ） and LY294002 plus BQ group （treated with 20 μmol/L LY294002 plus 3 μmol/L BQ）. The cell proliferation was measured with alamar blue dye assay. Western blot assay was used to detect the expression of p-Akt and Akt proteins and flow cytometer was used to observe the cell cycle. Results The cell proliferation rate and the cell proportion in the S, G2 phase of BQ group were 185.00%±30.00%, 48.23%±1.37% and 15.40%±1.21%, respectively, which were significantly higher than those （ 100.00%±0.00%, 42.47%±0.45% and 5.40%±0.40% ） of control group （P〈0.05）. But the cell proportion rate （36.37%±0.40%） in the G1 phase in BQ group was significantly lower than that （52.13%±0.75%） in control group （P〈0.05）. The expression level of p-Akt protein in BQ group was significantly higher than that in control group （P〈0.05）. The cell proliferation rate and the cell proportion in the S, G2 phase of LY294002 plus BQ group were 82.59%±15.00%, 42.03%±0.50% and 3.87%±0.47%, respectively, which were significantly lower than those of BQ group （P〈0.05）. But the cell proportion rate （54.43%±0.40%） in the G1 phase in LY294002 plus BQ group was significantly higher than that in BQ group （P〈0.05）. Conclusion The PI3FUAkt signal pathway may play an important role in the proliferation of HL-60 cells exposed to BQ.

关 键 词：苯醌类 细胞周期 1-磷脂酰肌醇3-激酶 蛋白激酶类 

分 类 号：R1[医药卫生—公共卫生与预防医学]