安多霖对微波辐射致大鼠心脏损伤的预防作用  

The protective effects of AduoLa Fuzhenglin on the heart injury induced by microwave exposure in rats

作　　者：张静[1] 彭瑞云[1] 任俊辉[1] 李静[1] 王水明[1] 高亚兵[1] 董霁[1] 赵黎[1] 刘曙晨[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100085

出　　处：《中华劳动卫生职业病杂志》2011年第5期367-370,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨安多霖对微波辐射致大鼠心脏损伤的预防作用。方法140只二级雄性Wistar大鼠随机分为5组：正常对照组、辐射对照组、0．75g·kg^-·d^-1安多霖预处理组、1．50g·kg^-·d^-1安多霖预处理组及3．00g·kg^-·d^-1安多霖预处理组。药物组每日1次灌胃给予安多霖溶液，连续给药2周。给药结束后即刻采用30mW／cm。微波辐射大鼠1次，辐射时间为15min。于辐射后7和14d，采用全自动生化检测仪检测血清中Ca^2＋含量、天冬氨酸转氨酶（AST）和肌酸激酶（cK）活力，用光学显微镜和电子显微镜观察心脏组织学和超微结构变化。结果微波辐射后7及14d，辐射对照组大鼠血清中Ca^2＋含量及CK、AST活力均升高，与正常对照组比较，差异均有统计学意义（P〈0．05或P〈0．01）；心肌纤维呈波浪状排列，内皮细胞核固缩、深染；线粒体肿胀、空化，闰盘结构模糊。0．75g·kg^-·d^-1安多霖预处理组上述指标变化与辐射对照组相似。1．50、3．00g·kg^-·d^-1安多霖预处理组大鼠上述指标与辐射对照组相比有所恢复，差异有统计学意义（P〈0．05），其中Ca^2＋含量及CK、AST活力变化与辐射对照组比较，差异有统计学意义（P〈0．05），心脏组织损伤趋于恢复。结论30mW／cm^2微波辐射可引起大鼠Ca^2＋含量及AST、CK活力异常及心脏组织学和超微结构损伤；1．50、3．00g·kg^-·d^-1安多霖对微波辐射致大鼠心脏损伤有预防作用。Objective To study the protective effects of AduoLa Fuzhenglin（ADL） on the heart injury induced by microwave exposure in rats. Methods One hundred forty male Wistar rats were divided randomly into 5 groups： control, microwave radiation, 0.75g· kg^-1·d^-1 ADL, 1.50g· kg^-1·d^-1 ADL and 3.00 kg^-1·d^-1 ADL pretreatment groups. Rats in three ADL pretreatment groups were administrated by ADL per day for 2w then exposed to 30 mW/cm^2 microwaves for 15 min. The left ventricle blood of rats was obtained at 7 d and 14 d after exposure to microwaves, and the blood Ca^2＋, AST and CK were detected with Coulter automatic biochemical analyzer, then the histological changes and ultrastructure of heart were observed under light and electron microscopes. Results At 7 d and 14 d after exposure to microwaves, the blood Ca^2 ＋, AST and CK concentrations significantly increased （P〈0.05 or P〈0.01 ） as compared with controls; Heart muscle fibers showed wavilness, endotheliocyte karyopyknosis, anachromasis; The mitochondria swelling and cavitation, intercalary dics blurred in radiation groups. The changes in 0.75 g·kg^-1·d^-1 ADL pretreatment group were similar to the radiation group, but in 1.50 g·kg^-1·d^-1 and 3.00g· kg^-1·d^-1 ADL pretreatment groups, above indexes of rats significantly reduced as compared with microwaves group （P 〈0.05）; also the blood Ca^2＋, AST, C K contents were significantly lower than those in microwave group （P〈0.05）; The heart showed a tendency to improve. Conclusion Microwave radiation （30 mW/cm^2） can cause the blood Ca^2＋, AST and CK turbulence, and heart injury in the histology and ultrastructure; ADL at the dosages of 1.50 g·kg^-1·d^-1 and 3.00 g·kg^-1·d^-1 has a protective effects on the heart injury induced by microwave in rats.

关 键 词：微波 天冬氨酸氨基转移酶 肌酸激酶 钙 心肌 

分 类 号：R285.5[医药卫生—中药学]