六神曲生品与炒制品的消化酶活力及胃肠动力比较  

Comparison of digestive enzyme activities and the effect on gastrointestinal motility of mice between unprocessed and processed Liushenqu

作　　者：张露蓉[1] 江国荣[1] 王斐[1] 王纯庠[1] 

机构地区：[1]江苏省苏州市中医医院中药临床药学实验室,苏州215003

出　　处：《中国临床药学杂志》2011年第3期148-150,共3页Chinese Journal of Clinical Pharmacy

基　　金：苏州市科技发展计划(编号SZD09150)

摘　　要：目的比较六神曲生品和炒制品在消化酶活力和促进胃肠动力方面的差异,为临床合理应用六神曲提供实验依据。方法淀粉酶活力应用碘量法测定,蛋白酶活力应用福林-酚试剂比色法测定;采用小鼠小肠推进实验方法评价六神曲生品和炒制品对胃肠蠕动的影响。结果六神曲生品的淀粉酶活力为(164.49±5.11)U·g^(-1),炒制后活力明显下降(P<0.01),为(19.86±0.65)U·g^(-1);六神曲生品的蛋白酶活力为(15.37±1.92)U·g^(-1),炒制后活力增强(P<0.01),为(22.74±0.96)U·g。。;六神曲生品和炒制品均能改善病理模型小鼠小肠的推进功能(P<0.05),且生品优于炒制品(P<0.05)。结论六神曲生品和炒制品均含有淀粉酶、蛋白酶成分和促进胃肠动力效应;六神曲生品中淀粉酶活力较高,而炒制后蛋白酶活力明显增高,临床可依据消化不良类型而分别合理使用。AIM To compare the digestive enzyme activities and the effect on gastrointestinal motor disorders in mice between unprocessed and processed Liushenqu for the clinical application of the Liushenqu. METHODS The amylase activity was decected by iodometry and the pmtease activity was detected by folin-phenol method. The effects on gastrointestinal motor disorders of unprocessed and processed Liushenqu were detected by intestinal content processing in mice. RESULTS The amylase activity was （164.49±5.11） U·g^-1in unprocessed Liushenqu and （19.86±0.65）U·g^-1 in processed Liushenqu. The pmtease activity was （ 15.37± 1.92） U·g^-1 in unprocessed Liushenqu, and （22.74 ±0.96） U·g^-1 in processed Liushenqu. Both unprocessed and processed Liushenqu improved the function of promoting the small intestinal propulsion on mice（ P 〈 0.05）, and the unprocessed Liushenqu was better than processed Liushenqu（ P 〈 0.05）. CONCLUSION Both unprocessed and processed Liushenqu have the amylase and the pmtease, and they are also effective on gastrointestinal motor disorders of mice. The amylase of unprocessed Liushenqu is more active, but the pmtease of processed Liushenqu is more active.

关 键 词：六神曲生品 六神曲炒制品 消化酶 胃肠动力 

分 类 号：R285[医药卫生—中药学]