口腔链球菌和变形链球菌耐酸相关基因ffh的同源性分析  

Analysis of homology of acid-resistant gen ffh in streptococcus oralis and streptococcus mutans

作　　者：唐玉香[1] 徐蓉蓉[1] 

机构地区：[1]南京大学口腔医学院口腔内科,南京210008

出　　处：《口腔医学》2011年第5期288-290,共3页Stomatology

摘　　要：目的检测口腔链球菌中是否存在耐酸相关基因,并对口腔链球菌和变形链球菌的ffh同源性进行分析。方法厌氧培养变形链球茵和口腔链球菌,试剂盒提取细菌基因组,根据GenBank基因库上发表的各种属ffh基因序列,从最保守区设计1对聚合酶链反应(PCR)引物,进行PCR。结果在以变形链球菌和口腔链球菌基因组为模板的PCR中,所获得的特异性片段与预期结果一致。结论口腔链球菌中存在耐酸相关ffh基因,且口腔链球菌和变形链球菌耐酸相关基因ffh同源性高。Objective To detect the gene ffh in streptococcus oralis,and to analyze the homology of ffh between streptococcus oralis and streptococcus mutans.Methods Streptococcus oralis and streptococcus mutans were cultured in anaerobic atmosphere and the genome was obtained with the alkaline method.A pair of primers was designed in the most reserved region of the sequences from GenBank database in order to obtain the unique polymerase chain reaction products.Results The unique product was found and obtained in streptococcus oralis and streptococcus mutans.Conclusions The gene ffh could be acquired from streptococcus oralis,and the homology of the acid-resistant gene ffh in streptococcus oralis and streptococcus mutans is high.

关 键 词：FFH 口腔链球菌 变形链球菌 聚合酶链反应 

分 类 号：R349.82[医药卫生—基础医学]