关龙胆抗肿瘤活性成分的研究  

Study on the anti-tumor active constituents of Gentiana scabra

作　　者：郭海凤[1] 朴惠顺[1] 

机构地区：[1]延边大学药学院,吉林延吉133000

出　　处：《华西药学杂志》2011年第3期204-207,共4页West China Journal of Pharmaceutical Sciences

基　　金：吉林省中医药管理局项目(08SYS-100);延边大学长白山生物资源与功能分子教育部重点实验室开放课题项目(2008第06号)

摘　　要：目的研究关龙胆Gentiana Scabra Bung中的抗肿瘤活性成分。方法采用色谱法分离关龙胆根中的化学成分,根据理化性质及波谱(MS、NMR、DEPT、1H-1H COSY、HSQC等)鉴定结构;采用MTT'法筛选分离所得化合物对宫颈癌细胞(He-la)、肝癌细胞(HepG-2)的抗肿瘤活性。结果从关龙胆中分离鉴定了10个单体化合物,分别为chirat-17(22)-en-3-one(Ⅰ)、β-谷甾醇(Ⅱ)、α-香树脂醇棕榈酸酯(Ⅲ)、chirat-16-en-3-one(Ⅳ)、chiratenol(Ⅴ)、3,4-dihydro-1H,6H,8H-naphtho[1,2-c:4,5-c',d']dipyr-ano-1,8-dione(Ⅵ)、胡萝卜苷(Ⅶ)、胆甾醇(Ⅷ)、龙胆苦苷(Ⅸ)、蔗糖(Ⅹ)。结论化合物Ⅲ、Ⅵ、Ⅷ、Ⅹ为首次从关龙胆中分离得到;化合物Ⅳ、Ⅴ、Ⅶ、Ⅹ对Hela和HepG-2细胞的增殖有一定的抑制作用。OBJECTIVE To study the anti-tumor active constituents from Gentiana scabra Bung.METHODS The chemical constituents were isolated from the Gentiana scabra Bung by ion exchange resin.Their structures were elucidated on the basis of physico-chemical properties and spectral data（MS,NMR,DEPT,1H-1H COSY,HSQC et al）.The anti-tumor active constituents on cervical（Hela） and livercancer（HepG-2） were selected by MTT.RESULTS Ten compounds were isolated and identified form the Gectiana scabra Bung which were chirat-17（22）-en-3-one（Ⅰ）,β-sistosterol（Ⅱ）,α-Amyrinpalmitate（Ⅲ）,chirat-16-en-3-one（Ⅳ）,chiratenol（Ⅴ）,3,4-dih-ydro-1H,6H,8H-naphtho[1,2-c：4,5-c′,d′]dipyrano-1,8-dione（Ⅵ）,β-daucosterol（Ⅶ）,cholesterol（Ⅷ）,gentiopicroside（Ⅸ）,and d-sucurose（Ⅹ）.CONCLUSION Compounds Ⅲ,Ⅵ,Ⅷ and Ⅹ were isolated from this plant for the first time.Compounds Ⅳ,Ⅴ,Ⅶ and Ⅹ exhibited moderate anti-tuomr effect against Hela and HepG-2 lines.

关 键 词：关龙胆 化学成分 宫颈癌细胞 肝癌细胞 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]