星点设计-效应面法优化醋酸地塞米松脂质纳米粒的制备工艺  

Optimization of the technological process to prepare Dexamethasone acetate lipid nanoparticles using central composite design-response surface methodology

作　　者：宋玉品[1] 王敏婷[1] 黄园[1] 汪世英[2] 

机构地区：[1]四川大学华西药学院靶向药物与释药系统教育部重点实验室,四川成都610041 [2]成都市第一人民医院,四川成都610041

出　　处：《华西药学杂志》2011年第3期264-267,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的优化醋酸地塞米松脂质纳米粒(DXMA-NLC)的制备工艺。方法采用薄膜超声法制备DXMA-NLC。以投药量、表面活性剂的浓度和大豆油的量为考察因素,以粒径和包封率为考察指标,根据星点设计原理安排实验,并用二项式拟合建立指标与因素之间的数学关系,经效应面法预测最佳工艺条件。结果采用15 mg DXMA、0.04 g·mL-1表面活性剂、100 mg大豆油制备DXMA-NLC,其粒径为156 nm、包封率为98.48%。结论按星点设计-效应面法优选的条件制备DXMA-NLC,得到与预测值相符的粒径与包封率,处方优化成功。OBJECTIVE To determine the optimized formulation of DXMA-NLC.METHODS The film-ultrasonic wave dissolving technique was chosen to prepare DXMA-NLC.The amount of DXMA,the concentration of surfactants and the quantity of soybean oil were defined as the independent variables while the size and entrapment efficiency were defined as the dependent variables.Experiments were done based on the theory of central composite design.The data were simulated using second-order polynomial equation.The optimal formulation was predicted by response surface methodology.RESULTS The optimized formulation was 15 mg DXMA,0.04 g·mL-1 surfactants,100 mg soybean oil.According to the optimized formulation,DXMA-NLC was prepared,resulting in a particle size of 156 nm and entrapment efficiency of 98.48%.CONCLUSION Central composite design could achieve the desired particle size and entrapment efficiency of DXMA-NLC.

关 键 词：醋酸地塞米松 脂质纳米粒 星点设计 效应面法 制备工艺 

分 类 号：R94[医药卫生—药剂学]