结肠癌术后早期肝转移的相关基因分析  

The Study on Genes Associated with Early-stage Liver Metastasis after Colorectal Cancer Surgery

作　　者：蔡相军[1] 王福荣[1] 王春喜[2] 王斌[1] 熊仁青[1] 刘彩刚[3] 冷晗[1] 

机构地区：[1]中国人民解放军第202医院普外科,沈阳110001 [2]中国人民解放军第301医院普外科,北京100039 [3]中国医科大学附属第一医院肿瘤科,沈阳110001

出　　处：《中国医科大学学报》2011年第6期549-552,共4页Journal of China Medical University

基　　金：沈阳市科学技术计划资助项目(071995)

摘　　要：目的探讨大肠癌早期肝转移的相关基因,为大肠癌肝转移的早期诊断提供理论依据。方法应用RT2 ProfilerTM PCRArray Human Tumor Metastasis(PAHS-028A)芯片,对原发灶和肝转移灶组织进行差异基因筛选。结果我们发现以下基因在大肠癌原发灶中表达明显高于肝转移结节:ACTB、APC、CTNNA1、NR4A3、MMP10、CTSL1、RB1、HPSE、ETV4、GNRH1、CDKN2A、KISS1R、IL8RB、ITGA7、ITGB3、DENR、RPSA,CXCR4、MYCL1、NME2、PNN、SMAD2,4、MMP11、SRC、RORB、SSTR2、SYK、TCF20、MMP3、TIMP2,3,4、TIPM1;以下基因肝转移灶表达明显高于原发灶:MMP9、FN1、CST7、CCL7、MGAT5。结论筛查大肠癌早期肝转移相关基因可以为合成大肠癌早期肝转移基因芯片提供依据。Objective To explore genes related to early-stage hver metastasis from colorectal cancer and provide the theoretical basis for the early diagnosis of liver metas ＇tasis from colorectal cancer. Methods The RT2 ProfilerTM PCR Array Human Tumor Metastasis （ PAHS- 028A ） chip was used to screen differential genes in the primary tumor and the liver metastases. Results It was found that the following genes were expressed at significantly higher levels in the primat7 tumors of colorectal cancer than in the hver me,static nodules：ACTB, APC , CTNNA 1, NR4A3 , MMP10, CTSL 1, RB1 , HPSE , ETV4 , GNRH I , CDKN2A , KISS 1 R, IL8B B , ITGA 7,1TGB3, DENR , RPSA , CXCR4 , MYCL1 , NME2 , PNN , SMAD2, 4 , MMPl l , SRC , RORB , SSTR2 , SYK , TCF20 , MMP3 , TIMP2 , 3 , 4 and TIPM I. The tbllowing genes were expressed higher in liver metastases than in the primary tumor： MMP9, FNI, CST7, CCL7 and MGAT5. Conclusion Screening for genes related to early-stage liver metas ＇tasis genes may provide basis for making gene chips of early-stage liver metastasis from colorectal cancer.

关 键 词：结肠癌 肝转移 转移相关基因 基因芯片 

分 类 号：R656.9[医药卫生—外科学]