HPLC测定固本咳喘片中白术内酯Ⅰ和白术内酯Ⅲ的含量  

Determination of Atractylenolide Ⅰ and Atractylenolide Ⅲ in Gubenkechuan Tablets by HPLC

作　　者：计耀成[1] 

机构地区：[1]丹江口市第一医院药学部,湖北丹江口442700

出　　处：《心血管病防治知识（学术版）》2011年第3期58-59,61,共3页Prevention and Treatment of Cardiovascular Disease

摘　　要：目的:建立同时测定固本咳喘片中白术内酯Ⅰ和白术内酯Ⅲ含量的方法。方法:采用HPLC法,AgilentHC-C18柱(4.6mm×250mm,5μm),流动相为甲醇:乙腈:0.2%磷酸溶液(52:5:43),流速为0.8ml·min^(-1),柱温:30℃,检测波长为222nm。结果:白术内酯Ⅰ和白术内酯Ⅲ分别在0.8～15.0μg·ml^(-1)(γ=0.9995)和1.7～33.0μg·ml^(-1)(γ=0.9997)浓度范围内均有良好的线性关系,平均加样回收率分别为98.22%,97.90%;RSD均小于2.0%。结论:本方法简便,快速,准确,重复性好,可用于该制剂的质量控制。Objective：To establish a method for the determination of atractylenolide I and atractylenolide Ⅲ in Gubenkechuan tablets. Methods：The reversed phase HPLC system consisting the Agilent HC-C18（4.6mm ×250mm,5μm）column,the mobile phase consisted of Methanol（52）-Acetonitrile（5）-0.2% H3PO4 （43）,the Ⅰ flow rate was 0.8ml · min^-1 , the column temperature was 30℃, The UV detector was set at 222nm. Results： The linear response range was 0.8-15.0μg ·ml^-1 （7=0. 9995）and 1.7-33.0μg ·ml^-1 （γ=0. 9997） ,respectively. The average recovery of atractylenolide Ⅰ and atractylenolide Ⅲ were 98.22% and 97.90%, respectively. Conclusions The assay demonstrated that the method had adequate and selectively to measure the concentrations of simple and accurate, repeatable, it can be applied in quantitative determination of atractylenolide Ⅰ and atractylenolide Ⅲ in Gubenkechuan tablets.

关 键 词：白术内酯Ⅰ 白术内酯Ⅲ 固本咳喘片 HPLC 

分 类 号：R286[医药卫生—中药学]