不同毒力疟原虫感染早期根治性治疗对免疫记忆形成影响的研究  

Effects of Radical Treatment in Early Phase of Primary Infection with Different Virulent Plasmodia on the Formation of Immune Memory

作　　者：刘英杰[1] 李莹[2] 潘艳艳[2] 朱晓彤[2] 刘军[2] 单风平[2] 曹雅明[2] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001 [2]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2011年第2期36-39,共4页Journal of Microbiology

摘　　要：为探讨不同毒力疟原虫感染早期根治性治疗对免疫记忆形成的影响,用致死型和非致死型约氏疟原虫感染DBA/2小鼠,感染后3 d进行根治性治疗,并于初次感染后90 d进行再感染。通过计数红细胞感染率和检测再感染前后不同时间点脾细胞中记忆性T、B细胞的百分含量后发现,再感染后2组小鼠均出现了短暂的低水平虫体血症;再感染前小鼠脾细胞中记忆性T、B细胞百分率均略高于正常鼠;但再感染后均出现了有意义的升高;在每一相同检测时间点,2组小鼠的虫体血症水平和记忆性T、B细胞百分率均无显著差异。这表明,不同毒力疟原虫感染早期的根治性治疗可使宿主产生水平相近的免疫记忆,再感染可促进免疫记忆的形成。Effects of radical treatment in early phase of primary infection with different virulent plasmodia on the forming of immune memory were investigated.DBA/2 mice were infected respectively with lethal P.y17XL and non-lethal P.y17XNL,and radically treatment was carried out on 3 d after the infection.And the mice were re-infected on 90 d after primary infection.The percentage of memorial T cell and B cell in spleen cells were counted and monitored through erythrocyte counting at different time points before and after the re-infection.It was found that the mice of both groups after the re-infection emerged transient plasmodiaemia at low level,and percentage of memory T cell and B cell in spleen cells of pre-reinfection mice were slightly higher than those in normal mice.However,significant increment was emerged in both groups after the reinfection.And in each same testing time point,the levels of plasmodiaemia,percentage of memory T cell and B cell of two groups of mice had no significant differences.These results suggested that radical treatment in early phase of primary infection might lead the hosts to produce similar level of immune memory when infected with different virulent plasmodia,and reinfection could promote the formation of immune memory.

关 键 词：不同毒力疟原虫 再感染 记忆性T细胞 记忆性B细胞 

分 类 号：R382.3[医药卫生—医学寄生虫学]