辛伐他汀对THP-1巨噬细胞源性泡沫细胞胆固醇代谢和SR-A表达的影响  

Effect of Simvastain on cholesterol metabolism and SR-A in THP-1 foam cell

作　　者：李晨光[1] 罗俊生[1] 冯旭[1] 霍小川[1] 关宁[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《山东医药》2011年第17期22-24,共3页Shandong Medical Journal

基　　金：国家自然科学基金(30971023);辽宁省自然科学基金(20092190)

摘　　要：目的探讨不同浓度辛伐他汀对THP-1巨噬细胞源性泡沫细胞胆固醇代谢和SR-A表达的影响。方法以50 nmol/L佛波酯诱导THP-1细胞分化成巨噬细胞,氧化型低密度脂蛋白(ox-LDL)诱导巨噬细胞建立泡沫细胞模型,通过不同浓度辛伐他汀干预后,油红O染色观察细胞内脂质堆积情况,高效液相色谱检测细胞内游离胆固醇和胆固醇酯含量,W estern b lot检测SR-A蛋白的表达。结果与ox-LDL组比较,辛伐他汀处理组巨噬细胞的油红O染色阳性细胞数和细胞内胆固醇酯含量均显著减少(P均<0.01),同时SR-A蛋白表达明显下降(P<0.01),呈剂量依赖性。结论辛伐他汀可抑制ox-LDL诱导的THP-1巨噬细胞泡沫化,可能与辛伐他汀抑制SR-A的表达,从而减弱巨噬细胞对ox-LDL的摄取有关。Objective To study the effect of Simvastatin on the expression of scavenger receptor class A（SR-A） and the cholesterol metabolism in THP-1 macrophage-derived foam cells.Methods The THP-1 cells were stimulated by phorbol 12-myristate 13-acetate（50 nmol/L） for 48 hours and transformed to macrophages.Macrophages were co-incubated with 100 mg/L oxidized low density lipoprotein（ox-LDL） and simvastatin.The cellular lipid accumulation was examined by oil red O staining.High performance liquid chromatography was used for qualitative and quantitative analysis of intracellular cholesterol and cholesterol esters.The expression of SR-A was detected by Western Blot.Results Compared to the ox-LDL group,oil red O-positive cells and cellular contents of cholesterol ester in Simvastatin group were greatly reduced（all P0.01）.The activity of SR-A was also significantly decreased（P0.01）.Simvastatin significantly attenuated these changes in a dose-dependent manner.Conclusions The datas suggest that Simvastatin is capable of inhibiting the formation of foam cells,which may be partly due to reduced SR-A expression and uptake of ox-LDL in macrophages.

关 键 词：辛伐他汀 脂蛋白类 LDL 单核细胞 泡沫细胞 清道夫受体 

分 类 号：R972.6[医药卫生—药品] R589[医药卫生—药学]