受体酪氨酸激酶抑制剂对兔OA模型软骨细胞NO的表达及软骨组织形态的影响  

The effects of genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats

作　　者：潘哲尔[1] 陈乃钱[1] 张纯武[1] 李驰[1] 黄小敬[1] 叶澄宇[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000

出　　处：《中医正骨》2011年第5期19-23,共5页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：温州市科技局科学研究基金(编号:NOY20090089)

摘　　要：目的:探讨受体酪氨酸激酶抑制剂genistein对兔OA模型软骨细胞NO的表达及软骨组织形态的影响,并探讨其可能的作用机制。方法:参照Hulth法建立兔骨关节炎模型,予以genistein干预后,分别按照0.1 mg.kg-1、0.2 mg.kg-1、0.3 mg.kg-1、0.4 mg.kg-1、0.5 mg.kg-1的给药剂量进行分组,行手术侧关节腔内注射,通过测定各组亚硝酸盐含量检测关节腔内NO的表达水平,同时进行软骨组织形态学检查。结果:0.3 mg.kg-1、0.4 mg.kg-1、0.5 mg.kg-1浓度genistein干预组关节腔内NO的表达水平明显低于对照组,差异具有统计学意义(P<0.05);对软骨组织行H.E/SOFG染色显示,在造模后第4周、第8周与第12周时,genistein干预组与对照组相比,组织损伤均明显减轻,Mankin评分均明显下降,差异具有统计学意义(P<0.05)。结论:受体酪氨酸激酶抑制剂genistein在体内能减少骨关节炎软骨细胞NO的表达,对于延迟关节软骨在组织形态水平的退变是有效的,但是尚不能最终阻止软骨退变发生发展的进程。Objective：To investigate the effects of recePtor tyrosine kinase inhibitor genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats,thus try to exPress the Probable mechanisms involved.Methods：According to Hulth method,we built rat OA model and the exPression levels of NO was detected by Griess method and the change of ultra-microstructure of cartilage was observed.Results：ComPared with control grouP,less levels of NO were detected after the intervention of genistein（0.3 mg·kg-1、0.4 mg·kg-1 and 0.5 mg·kg-1）（P0.05）.At 4、8 and 12 weeks after oPeration,cartilage sections made and stain with HE/SOFG stain showed less injury,and Mankin score were significantly decreased in intervention grouPs comPared with control grouP（P0.05）.Conclusion：Genistein can reduced the exPression of NO in-vivo.The results of Mankin score and HE/SOFG stain showed that genistein Partly delayed the observation of ultra-microstructure of cartilage exPlored the alleviation of the ultra-microstructure damage of cartilage after injury.

关 键 词：骨关节炎 软骨细胞 受体酪氨酸激酶抑制剂 一氧化氮 

分 类 号：R684.3[医药卫生—骨科学]