CdSe@ZnS量子点在果蝇及其幼虫体内的分布  

Distribution of CdSe@ZnS QDs in adult drosophila and their stage-1 larva

作　　者：刘慧[1,2] 汪冰[1] 王卓[2] 李明[1] 康艳杰[1,3] 秘晓林[1] 余笑寒[4] 丰伟悦[1] 

机构地区：[1]中国科学院高能物理研究所纳米生物效应与安全性重点实验室和核分析技术重点实验室,北京100049 [2]烟台大学环境与材料工程学院,烟台264005 [3]郑州大学化学系,郑州450001 [4]中国科学院上海应用物理研究所,上海201800

出　　处：《核技术》2011年第6期415-418,共4页Nuclear Techniques

基　　金：国家自然科学基金(10905064;10975148和20805048);科技部973项目(2011CB933400);中国科学院知识创新重大项目(KJCX3.SYW.N3)资助

摘　　要：用同步辐射微束X射线荧光(μ-SRXRF)技术结合激光扫描共聚焦显微成像(LSCM)技术研究了经CdSe@ZnS量子点口暴露的果蝇成虫及其子代一期幼虫体内的微区分布。μ-SRXRF分析表明,果蝇成虫摄取CdSe@ZnS量子点后,果蝇成虫胃肠道组织中有Cd、Zn和Se元素的富集,说明量子点经果蝇口服暴露后主要富集在肠道和生殖道部位。LSCM观察表明,CdSe@ZnS量子点暴露后成虫所产一期幼虫的生殖部位(精巢)有明显的荧光增强信号,表明量子点暴露能够经果蝇母体转运到子代。μ-SRXRF分析技术为纳米颗粒在活体内转运研究提供了很好的分析手段。In this work,CdSe@ZnS quantum dots（QDs） distributed in the QDs-exposed adult drosophila and their stage-1 larva were characterized by synchrotron radiation micro X-ray fluorescence（μ-SRXRF） and laser scanning confocal microscope（LSCM）,respectively.The CdSe@ZnS QDs were exposed via the mouth to the adult drosophila.The μ-SRXRF elemental mapping of Cd,Se and Zn revealed that the elements were concentrated in the intestinal and reproductive system,indicating that QDs could accumulate in the intestinal and reproductive system.Obvious fluorescence signals were observed in the testis of stage-1 larva by LSCM technique.The results indicate the QDs via oral exposure could induce potential impact on placenta translocation and reproductive system.The results show that μ-SRXRF analysis is a novel method for the investigation of translocation of nanoparticle in the living organism.

关 键 词：μ-SRXRF CdSe@ZnS量子点 果蝇 微区分布 

分 类 号：O434.14[机械工程—光学工程]