He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复  

Ramage Repair for Enhanced Ultraviolet-B Radiation on Antioxidant System of Arabiodopsis by He-Ne Laser

作　　者：姜晓燕[1] 高丽美[1] 李永峰[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《光子学报》2011年第5期712-717,共6页Acta Photonica Sinica

基　　金：国家自然科学基金(No.30671061);山西省自然科学基金(No.2008011059-1)资助

摘　　要：以培养6周左右的拟南芥为材料,采用UV-B辐射(剂量1 KJ/m2/d)和He-Ne激光器(波长632.8 nm,输出功率5 mW.mm2,辐照时间60 s)对材料进行处理,分成CK(没有经过UV-B或激光辐照)组、B(UV-B辐射)组、BL(UV-B和激光复合处理)组和L(激光辐照)组4个不同处理组.结果表明:增强的UV-B辐射拟南芥幼苗导致MDA(Malondialdehyde)、超氧阴离子含量升高,GSH(Glutathione)含量降低,PAL(phenylalanine ammomia-lyase)、CAT(catalase)和APX(ascorbate peroxidase)活性升高,SOD(supemxide dismutas)活性降低.单独He-Ne激光处理使MDA、超氧阴离子含量降低,GSH的含量升高,SOD、APX、CAT的活性升高,PAL的活性降低.UV-B辐射后再用He-Ne激光进行后处理,发现与单独UV-B辐照处理相比,MDA、超氧阴离子含量降低,GSH含量升高,SOD、APX、CAT的活性升高了,PAL的活性降低了.因此激光在一定程度上提高了拟南芥叶片抗氧化能力,在此基础上讨论了其可能的形成机理.Taking Arabidopsis trained about six weeks as the material,UV-B radiation（1 kJ/m2/d） and He-Ne laser irradiation（wavelength 632.8 nm output power 5 mW·mm2,irradiation time 60 s） were used to process the material.The Arabidopsis were divided into four treatment groups including CK（not treated）,L（He-Ne laser）,B（UV-B）,and BL（UV-B ＋He-Ne laser）.The experimental results show that enhanced UV-B increases the contents of MDA,O.-2and the activities of PAL,CAT,APX,while SOD activity and the content of GSH decrease.But He-Ne laser irradiation reduces the contents of MDA and O.-2,the PAL activity,and increases SOD activity,APX activity,CAT activity,the content of GSH.In contrast to B group,both enhanced UV-B and He-Ne laser have similar results which indicated by He-Ne laser irradiation.The results prove that laser can improve the ability of antioxidant system in Arabidopsis.

关 键 词：HE-NE激光 UV-B辐射 拟南芥 抗氧化系统 

分 类 号：Q945[生物学—植物学]