Bt野生菌Q1杀虫晶体蛋白及基因型分析  

ANALYSIS OF INSECTICIDAL CRYSTAL PROTEIN AND ITS CRY-TYPE GENES OF BACILLUS THURINGIENSIS

作　　者：李先军[1] 李强[1] 杜超[1] 王树艳[2] 

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2011年第2期124-126,共3页Journal of Chengde Medical University

摘　　要：目的:分析我国千山地区森林地带土壤中分离的野生菌Q1的32类杀虫晶体蛋白基因类型和表达蛋白。方法:利用PCR-RFLP鉴定体系和SDS-PAGE蛋白分析方法,以JM110为受体菌、PMD-18T为克隆载体对cry7基因的同源区段进行克隆测序。结果:Q1同时含有cry1、cry7、cry16基因,同时表达了130kD、90kD和60kD蛋白。电镜观察显示晶体形状多为菱形或长菱形。测序后与模式基因比对相似性均在89%以上。结论:该实验为克隆cry7全长基因奠定了研究基础,丰富了菌株及基因资源,在资源积累方面具有重要意义。Objective：To analyze the gene type and expressed proteins of 32 kinds insecticidal crystal protein in Bacillus thuringiensis wild strain Q1 isolated from forest soil of Qianshan area.Methods：PCR-RFLP identification system and SDS-PAGE protein analytical method were used in this study.The homologous sequence of cry7 gene was cloned and sequenced taken JM110 as recipient bacterium and PMD-18T as cloning vector.Results：Q1 contained three cry-type genes：cry1,cry7 and cry16,and encoded 130,90,60 kD protein at the same time.The electron microscope showed rhombus shape crystal.Compared with model gene,cry7 homologous sequence homology was more than 89%.Conclusions：This research enriches strains and genetic resources,and has important significance in accumulation of resources.

关 键 词：苏云金芽孢杆菌 杀虫晶体蛋白 CRY基因 RCR-RFLP 

分 类 号：Q785[生物学—分子生物学]