蛋白基因产物9·5和泛素在IgA肾病中的表达  

Expression of protein gene product 9.5 and ubiquitin in IgA nephropathy

作　　者：贾宁[1] 王汉[2] 彭莉[1] 

机构地区：[1]中山大学附属第五医院肾内科,珠海519000 [2]中山大学附属第五医院神经外科,珠海519000

出　　处：《中国实用医药》2011年第14期28-29,共2页China Practical Medicine

摘　　要：目的观察蛋白基因产物9·5(PGP9·5)和泛素(ubiquitin)在IgA肾病(IgAN)患者肾组织的表达及其与临床病理的关系,探讨其在IgAN病变的作用。方法 53例IgAN患者分为单纯血尿组(A组)及混合蛋白尿、血尿组(B组),免疫组织化学和免疫双染色法观察PGP9·5和泛素在IgAN肾组织中的表达。结果免疫组织化学显示在IgAN肾组织中,PGP9·5和泛素均有表达,主要在上皮细胞(壁上皮细胞、特别是小管上皮细胞)及新月体表达。免疫组化双染色显示:泛素分布更广泛,PGP9·5表达比泛素表达稍强,按Lee氏分级的级别增加逐渐增高,免疫组织化学半定量评分显示增生肥厚病变部位及新月体表达明显强于正常和纤维化/萎缩的部位(P<0·01),PGP9·5及泛素的表达与患者24h尿蛋白定量及病理分级成正相关。结论 PGP9·5及泛素可能在IgAN病变及进展中发挥了重要作用。Objective To explore the expression of protein gene product 9.5（PGP 9.5） and ubiquitin in the renal tissue of patients with IgA nephropathy,to study the role of protein gene product 9.5 and ubiquitin on IgA nephropathy and relationship between PGP 9.5,ubiquitin express and clinicopathologicol features of I gAN.Methods Immunohistochemistry and double stain were applied to detect the expression of protein gene product 9.5 and ubiquitin in 53IgA nephropathy patients.Results Immunohistochemistry showed PGP 9.5 and ubiquitin were expressed in IgAN,especial in epithelial cells and crescentic,with the strongest positivity in hypertophic condition and weak positivity in atrophic condition was correlated（P0.01）,the expression of PGP 9.5 and ubiquitin protein in IgAN was correlated with pathological grading,24 hours urineproteins.Conclusion PGP 9.5 and ubiquitin may play an important role in IgAN.

关 键 词：IGA肾病 蛋白基因产物9·5 泛素 24 蛋白尿 

分 类 号：R692.3[医药卫生—泌尿科学]