不同钙效应剂对长春花光合特性的影响  

Effects of Different Calcium Reagents on Photosynthesis Characteristics of Catharanthus roseus

作　　者：孙金春[1,2] 张扬欢[1,2] 温泉[1,2] 张楠[1,2] 唐娟[1,2] 吴能表[1,2] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2011年第6期74-78,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30500041);中央高校基本科研业务费专项资金资助项目(XDJK2009C195)

摘　　要：研究了细胞质膜钙通道阻断剂氯化镧(LaCl3)、胞内IP3通道阻断剂肝素(Heparin)、胞内CaM活性抑制剂三氟啦嗪(TFP)对长春花光合作用的影响,结果发现:Ca2+通道阻断剂处理后长春花叶片净光合速率(Pn)、PSII最大量子产量(Fv/Fm)、PSⅡ实际量子产量(Yield)、电子传递速率(ETR)、光化学淬灭系数(qP)均下降,非光化学淬灭系数(qN)及Chla、Chlb、Chla+Chlb含量上升,表明Ca2+通道阻断剂对长春花的光合作用有较大影响,且不是通过加速叶片光合色素降解或抑制其合成来实现抑制叶片的光合能力;其中Heparin处理的长春花叶片相关参数变化幅度最大,表明胞内钙库通过IP3通道释放的Ca2+在长春花叶片光合作用过程中发挥了更积极的作用.The effects of LaCl3（a plasmalemma calcium channel blocker）,herpain（an intracellular IP3 channel blocker） and trifluoperazine（an intracellular CaM activity inhibitor） on the photosynthesis of Catharanthus roseus were researched.All the calcium channel blockers studied decreased Pn（net photosynthesis rate）,maximal PSII quantum（Fv/Fm）,PSII quantum yield（Yield）,relative electron transport rate（ETR） and photochemical quenching（qP） of C.roseus and increased its non-photochemical quenching（qN）,chlorophyll a concentration,chlorophyll b concentration and chlorophyll a＋b.It is,therefore,concluded that the calcium channel greatly influenced the photosynthesis of C.roseus by a special way other than promoting the degradation of photosynthetic pigments or the inhibition of their synthesis to inhibit the photosynthesis of C.roseus.Herpain affected photosynthesis of C.roseus most significantly,which means the calcium released by intracellular calcium pool through the IP3 channel plays an important role in the photosynthesis of C.roseus.

关 键 词：钙效应剂 光合特性 长春花 叶绿素荧光 

分 类 号：Q26[生物学—细胞生物学]