正常人前列腺组织PSP mRNA的表达  

mRNA EXPRESSION OF PSP mRNA IN NORMAL PROSTATE TISSUE

作　　者：刘丽[1] 刘建香[1] 刘庆鑫[1] 刘立忠[1] 王颖[1] 孟岩[1] 谢宝树[1] 冷爱军[1] 苏旭 刘树铮 

机构地区：[1]空军总医院 [2]白求恩医科大学预防医学院放射生物教研室

出　　处：《解放军医学杂志》1999年第4期265-267,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：用ＲＴ－ＰＣＲ和ｃＤＮＡ序列测定法分析５名正常中国人前列腺组织中前列腺分泌蛋白（ＰＳＰ）ｍＲＮＡ的表达。结果表明，在这５名正常前列腺组织中均同时存在ＰＳＰ９４和ＰＳＰ５７ｃＤＮＡ，且基因序列与文献报道的从前列腺癌和增生前列腺组织中获得的完全一致。这可能对ＰＳＰ进一步的基础研究和应用有一定的意义。Prostate secretory protein(PSP) in human seminal plasma is an androgen independent protein synthesized and secreted by the prostate. There are two types of mature PSP generated by alternative mRNA splicing: one is PSP94 containing 94 amino acids residues, the other is PSP57 containing 57 amino acids.It has been reported that expression of PSP94 and PSP57 mRNA is found in prostate carcinoma and benign prostatic hyperplasia(BPH), but PSP57 cDNA is not found in prostate tissue from normal adult. This paper analyzes the expression of PSP mRNA in prostate tissue from five normal Chinese by RT PCR and cDNA sequence determination. The results show that both PSP94 and PSP57 cDNA are found in prostate tissue from these five individuals. Furthermore, their gene sequences are consistent with the formerly reported sequences. Perhaps the finding is significant for further basic research and clinical applications of PSP.

关 键 词：前列腺分泌蛋白 MRNA 前列腺组织 

分 类 号：R339.21[医药卫生—人体生理学] Q75[医药卫生—基础医学]