生长因子网络调节对骨形成作用的研究——Ⅵ.多种生长因子联合应用对人胚成骨样细胞增殖及分化的影响  

The Effects of Combined Use of Multiple Growth Factors on Proliferation and Differentiation of Human Osteoblast like Cells

作　　者：田卫东[1] 高贤华[1] 王大章[1] 陈伟辉[1] 

机构地区：[1]华西医科大学口腔医学院颌面外科,成都610041

出　　处：《华西医科大学学报》1999年第3期271-273,共3页Journal of West China University of Medical Sciences

基　　金：国教委博士学科点专项基金; CMB基金

摘　　要：研究转化生长因子（（ Ｔ Ｇ Ｆβ）、胰岛素样生长因子Ⅱ（ Ｉ Ｇ ＦⅡ）、碱性成纤维细胞生长因子（ｂ Ｆ Ｇ Ｆ）及血小板衍生性生长因子（ Ｐ Ｄ Ｇ Ｆ）等四种生长因子中，三种生长因子交互应用和四种生长因子联合应用对人胚成骨样细胞增殖与分化的影响。在不同的实验期间检测成骨样细胞３ Ｈ胸腺嘧啶脱氧核苷（３ Ｈ Ｔｄ Ｒ）、３ Ｈ脯氨酸（３ Ｈ Ｐｒｏｌｉｎｅ）的掺入量和碱性磷酸酶的含量。结果显示：三种和四种生长因子交互联合应用，对人胚成骨样细胞增殖与分化有明显的促进作用。预示具有协同作用的生长因子联合应用，可提高其促进骨形成的生物学效应，可能成为临床治疗骨疾病和修复骨缺损的一种新途径。Four growth factors PDGF, bFGF, IGF Ⅱ and TGF β, which is belived to have biological effects on bone cells, were examined in this study. The aim was to assess the effects of combinations of three and four growth factors on the proliferation and differentiation of osteoblast like cells. The incorporation of 3H TdR, 3H Proline of osteoblast like cells and alkaline phosphatase content of the cells were tested. The results showed the combinations of three and four growth factors stimulated the synthesis of DNA, collagen and ALP of osteoblast like cells. These four growth factors interacted synergistic ally. The combinations of three and four growth factors showed stronger promoting effect on osteoblast like cells, compared with the combinations of two growth factors. These findings suggest that the combined use of growth factors be a potential way of bone defect reconstruction and treatment of human bone disease.

关 键 词：生长因子 人胚成骨样细胞 骨形成 细胞增殖 

分 类 号：R681.02[医药卫生—骨科学]