妊高征患者胎盘中一氧化氮合酶含量的变化及其意义  

Changes of Placental Nitric Oxide Synthase in Patients with Pregnancy Induced Hypertension

作　　者：周容[1] 李维敏[1] 曹泽毅[1] 肖邦良 刘淑芸[1] 熊庆[1] 程薇波 

机构地区：[1]华西医科大学附属第二医院妇产科,成都610041 [2]公共卫生学院毒理实验室

出　　处：《华西医科大学学报》1999年第3期312-314,共3页Journal of West China University of Medical Sciences

摘　　要：为探讨胎盘中一氧化氮合酶（ Ｎ Ｏ Ｓ）在妊高征发病中的作用，采用免疫组化 Ａ Ｂ Ｃ法分析了３２例妊高征患者胎盘和３２例正常足月孕胎盘中，内皮型一氧化氮合酶（ｅ Ｎ Ｏ Ｓ）和诱导型一氧化氮合酶（ｉ Ｎ Ｏ Ｓ）含量的变化情况。结果：①正常足月孕胎盘和妊高征患者胎盘中均存在ｅ Ｎ Ｏ Ｓ和ｉ Ｎ Ｏ Ｓ抗原，两者均分布于绒毛血管内皮细胞和合体滋养细胞胞浆内；②妊高征患者胎盘中ｅ Ｎ Ｏ Ｓ含量显著低于正常足月孕者（ Ｐ＜ ０．０００５），且轻度妊高征患者胎盘ｅ Ｎ Ｏ Ｓ含量显著高于中度及重度患者（ Ｐ＜ ０．０２５， Ｐ＜ ０．００５）。妊高征患者的血压与 ｅ Ｎ Ｏ Ｓ含量之间有显著的负相关（ｒ＝ － ０．６０１１， Ｐ＜ ０．０００５）；③妊高征患者胎盘ｉ Ｎ Ｏ Ｓ含量与正常足月孕者无显著差异。轻度与中度、轻度与重度以及中度与重度妊高征患者胎盘ｉ Ｎ Ｏ Ｓ含量均无显著差异。妊高征患者的血压与ｉ Ｎ Ｏ Ｓ含量之间无相关性。以上说明：妊高征患者胎盘中ｅ Ｎ Ｏ Ｓ含量的减少与其发病有关。The objective of this study was to determine whether the expression of placental endothelial nitric oxide synthase (eNOS) and/or inducible nitric oxide synthase (iNOS) are changed in pregnancy induced hypertension (PIH). The placentas of 32 patients with PIH and 32 normal pregnancies were studied by immunohistochemistry (Avidin Biotin Complex method). The results showed there were eNOS and iNOS antigens in placental tissues from PIH and normal pregnancy. They were all localized in placental villi and syncytiotrophoblast cells. The expression of placenta eNOS decreased significantly in patients with PIH as compared with that of normal pregnancy. The expression of placenta eNOS in mild PIH was higher than that of moderate or severe PIH ( P <0.025, P <0.005, respectively). There was a negative correlation between blood pressure and the expression of eNOS in placenta( P <0.0005). There was no significant difference between the expression of iNOS in placenta with PIH and that in normal pregnancy. Also, there was no significant difference of the expression of iNOS in placenta between the mild and moderate PIH, or the mild and severe PIH. Furthermore, there was no correlation between blood pressure and the expression of iNOS in placenta with PIH. It is concluded that the decrease of the expression of eNOS in placenta may be associated with the pathogenesis of PIH.

关 键 词：一氧化氮合酶 妊娠高血压 胎盘 

分 类 号：R714.246[医药卫生—妇产科学]