Tyrosinase-modified carbon felt-based flow-biosensors:The role of ultra-sonication in shortening the enzyme immobilization time and improving the sensitivity for p-chlorophenol  

作　　者：Yasushi Hasebe 

机构地区：[1]Department of Material Science and Engineering,Graduate School of Engineering,Saitama Institute of Technology,1690 Fusaiji,Fukaya,Saitama 369-0293,Japan [2]Department of Life Science and Green Chemistry,Saitama Institute of Technology,1690 Fusaiji,Fukaya,Saitama 369-0293,Japan

出　　处：《Journal of Environmental Sciences》2011年第6期1038-1043,共6页环境科学学报（英文版）

基　　金：supported in part by the Open Research Center Project of the Ministry of Education,Science and Culture of Japan

摘　　要：Tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min.The resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates（catechol,p-chlorophenol（p-CP）,p-cresol,phenol etc.）.Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at-50 mV vs.Ag/AgCl.Compared with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment,we succeeded in（1） shortening the enzyme immobilization time from 16 hr to 10 min,（2） enhancing the sensitivity of p-CP,and（3） improving the operational stability of p-CP.The ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.Tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min.The resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates（catechol,p-chlorophenol（p-CP）,p-cresol,phenol etc.）.Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at-50 mV vs.Ag/AgCl.Compared with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment,we succeeded in（1） shortening the enzyme immobilization time from 16 hr to 10 min,（2） enhancing the sensitivity of p-CP,and（3） improving the operational stability of p-CP.The ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.

关 键 词：enzyme immobilization under ultrasonic treatment flow-biosensor carbon felt TYROSINASE phenolic compounds 

分 类 号：O657.1[理学—分析化学]