丹参单体IH764-3对rhIL-8诱导的人嗜中性白细胞趋化游走过程的影响  

Effects of IH764-3 on chemotactic migration of neutrophils induced by rhIL-8

作　　者：陈克奇[1] 周宇[1] 邢海燕[1] 朱文云[1] 金永娟[1] 

机构地区：[1]中国医学科学院

出　　处：《中国病理生理杂志》1999年第9期781-783,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金!39470820

摘　　要：目的和方法：采用两种体外细胞趋化游走实验体系及间接免疫荧光等方法，观察丹参单体（IH764－3）对rhIL－8诱导的人嗜中性白细胞（neutrophils，Neu）跨聚碳酸酯（PC）膜和人脐静脉内皮细胞（humanumbilicalveinendothelialcells，HUVEC）单层趋化游走过程的影响，以分析IH764－3的抗炎作用机制。结果：与对照组比较、IH764－3对rhIL－8诱导的Neu跨PC膜趋化游走（P＜0．01）和跨HUVEC单层趋化游走（P＜0．01）均有明显地抑制作用。这种抑制作用是IH764－3抗炎作用机制之一，与对照组比较、IH764－3对rhIL－8诱导NeuCD11b表达（P＜0．01）及Neu与HUVEC单层粘附（P＜0．01）均有明显抑制作用。结论：这种抑制作用是IH764－3抑制Neu趋化游走的深层机制。AIM and METHODS:Two in vitro cells migration test (a 48 well micro chemotaxis chamber system and a Transwell coculture system) and indirect immunofluorenscence test were used to gain a deep insight into the effects of IH764 -3 (profocatechuic aldehyde), one of monomers extracted from Radix Salviae Miltiorrhizae, a Chinese plant, on transpolycarbonate membrane and trans-human umbilical vein endothelial cells (HUVEC) monolayer chemotactic migration of human neutrophils (Neu) induced by human recombinant interleukin - 8(rhIL - 8); to analyse the mechanisms of IH64-3 antiinflammatory action. RESULTS: Compared with control group, IH764 - 3 inhibits significantly both transpolycarbonate membrane migration of Neu(P < 0.001 ) and trans -HUVEC monolayer migration of Neu (P < 0.01) induced by rhIL - 8. The inhibition is thought to be one of the mechanisms for IH764 - 3 antiinflammatory action. On the other hand, compared with control conditions, IH764-3 inhibits both CD11b expression on Neu induced hy rhIL - 8(P < 0.01 ) and rhIL-8 induced Neu adhesion to HUVEC monolayer (P <0.01 ) significantly. CONCLUSION: The inhibition is thought to be an underlying mechanisms for IH764- 3 antiinflammatory action.

关 键 词：丹参 单体IH764-3 rhIL-8 NEU PC膜 HUVEC 

分 类 号：R285.5[医药卫生—中药学]