同种MHC基因胸腺转移诱导小鼠心肌移植耐受  

Transfer of allogenic MHC gene into thymus by retroviral vector induces tolerance

作　　者：李锦华[1] 唐皓[2] 余学清[1] 陈永雄[1] 张仕光[1] 

机构地区：[1]中山医科大学附属第一医院肾内科,广州510080 [2]中山医科大学附属第一医院急诊科,广州510080

出　　处：《中国病理生理杂志》1999年第9期793-796,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金!39570343

摘　　要：目的：通过胸腺内注射表达外源性主要组织相容性抗原复合物（MHC）抗原质粒PXN（N2－B19－H－2Kb），诱导异基因小鼠心肌移植耐受。方法：给BALB／C小鼠胸腺内注射质粒PXN（N2－B19－H－2Kb），将外源性的编码C57BL／6小鼠MHCI类抗原的H－2KbcDNA转移到BALBC／C小鼠胸腺，2周后行C57BL／6小鼠心肌移植。用聚合酶链反应（PCR），反转录聚合酶链反应（RT－PCR），单克隆抗体免疫萤光染色流式细胞仪检测BALB／C小鼠胸腺细胞DNA，mRNA和MHC蛋白质表达。结果：BALB／C小鼠胸腺细胞表面有外源性MHC分子表达，转染效率为5．1％，胸腺内注射质粒PXN（N2－mg－H－2Kb）能明显延长移植小鼠心肌的存活时间，平均17d，对照为8d（P＜0．01）。结论：同种MHC基因转移至受体鼠胸腺可以诱导特异性的免疫耐受。AIM: A mice myocardium allograft tolerence model was constructed by intrathymic injection of retroviral of retroviral vector PXN (N2 - B19 - H -2Kb) to express allogenice major histocompatibility complex(MHC). MEHTODS: Retroviral vector PXN(N2 - B19 - H - 2Kb)was injected into BALA/C thyms directly to express MHC of C57BL/6(H - 2Kb); after 14 dsys, myocardium of C57BL/(H - 2Kb) mice was transplantated into RALA/C mice; and the polymemrase chain reaction (PCR), the reverse transcription polymerase chain reaction (RT- PCR) and flow cytometly assays detected DNA, mRNA and MHC protem expression in thymocytes. RESULTS: Allogenic MHC gene had been integrated intO the chromosomal DNA of BALA/C thymus and expressed stably; It induced meuse heaxt allograf tolerance, 17days us 8 day; P < 0. 01. CONCLUSION: Allogenic MHC gene transferring into recipient thymus directly could induce allogenice mice tolerance.

关 键 词：基因转移 免疫耐受 心脏移植 MHC基因 小鼠 

分 类 号：R654.2[医药卫生—外科学] R392.4[医药卫生—临床医学]