乳汁HBV-DNA定量时标本处理的优选及临床应用  

Optimization and clinical application of treatment of specimens in HBV-DNA quantitation

作　　者：方有兵[1] 黄晨艳[2] 刘贵育[1] 

机构地区：[1]安徽医科大学第一附属医院检验科,安徽合肥230022 [2]合肥市妇幼保健院医务科

出　　处：《中国妇幼保健》2011年第17期2664-2666,共3页Maternal and Child Health Care of China

基　　金：安徽省合肥市科技局医学应用性研究项目〔HF200802642〕

摘　　要：目的:选择灵敏度高、重复性较好的乳汁HBV-DNA定量标本提取方法应用于乙肝携带产妇的乳汁检测。方法:选择14例乙肝"大三阳"产妇乳汁,每份分为乳汁原标本,乳汁离心后中层,乳汁离心后下层沉淀混合液3组,分别提取HBV-DNA并检测含量,用秩和检验分析结果的差异;用乳汁离心后中层对316例乙肝产妇检测HBV-DNA含量。结果:乳汁离心后中层清液灵敏度高于乳汁原样本,乳汁离心后中层清液重复性高于下层沉淀混合液。在316例乙肝产妇中,血清HBsAg+、HBeAg+、HBcAb+组,其乳汁HBV-DNA阳性率为77.60%;血清HBsAg+,HBeAb+,HBcAb+组其乳汁HBV-DNA阳性率为0.73%,血清HBsAg+,HBeAg+组乳汁HBV-DNA阳性率为80.00%,血清HBsAg+和HBcAb+组乳汁HBV-DNA阳性率为5.17%,其余组别乳汁HBV-DNA阳性率皆为0。结论:乙肝携带者产妇的乳汁可用于评估母乳喂养安全性。在乙肝产妇乳汁中,以其血清HBsAg+、HBeAg+、HBcAb+者和血清HBsAg+、HBeAg+者母乳喂养小儿被传染乙肝病毒的风险较大。Objective：To choose a method with high sensitivity and good repeatability of drawing HBV-DNA quantitative specimens from colostrum used for colostrum detection of lying-in women with hepatitis B.Methods：14 colostrum specimens from 14 lying-in women carrying positive HBsAg,HBeAg and HBcAb were selected,and every colostrum specimen was divided into 3 groups： original specimen,centrifugal specimen at middle level and centrifugal sediment at low level,then HBV-DNA was extracted respectively and the content was detected,rank sum test was used to analyze the difference of results;centrifugal specimens at middle level were used to detect the content of HBV-DNA in 316 lying-in women with hepatitis B.Results：The sensitivity of centrifugal specimen at middle level was higher than that of original specimen,and the repeatability of centrifugal specimen at middle level was higher than that of centrifugal sediment at low level.Among 316 lying-in women with hepatitis B,in positive serum HBsAg,HBeAg and HBcAb group,the positive rate of HBV-DNA in colostrum was 77.60%;in positive serum HBsAg,HBeAb and HBcAb group,the positive rate of HBV-DNA in colostrum was 0.73%;in positive serum HBsAg and HBeAg group,the positive rate of HBV-DNA in colostrum was 80.00%;in positive serum HBsAg and HBcAb group,the positive rate of HBV-DNA in colostrum was 5.17%;in other groups,the positive rates of HBV-DNA in colostrum were all 0.Conclusion：The colostrum of lying-in women with hepatitis B can be used to assess the safety of breastfeeding.The infants fed with breastfeeding of colostrum from the lying-in women with positive serum HBsAg,HBeAg and HBcAb or positive serum HBsAg,HBeAg have high risk of hepatitis B virus infection.

关 键 词：乳汁 HBV-DNA检测 离心沉淀 乙肝产妇 母乳喂养 

分 类 号：R512.61[医药卫生—内科学]