利用农杆菌介导法获得转cry2A抗虫基因水稻的研究  

Development of Japonica Rice with Insect-resistant Gene Cry2A by Agrobacterium-mediated Transformation

作　　者：吴振映[1] 柳絮[2] 王文英[2] 姚方印[2] 刘开启[1] 

机构地区：[1]仲恺农业工程学院,广东广州510225 [2]山东省农业科学院高新技术研究中心,山东济南250100

出　　处：《山东农业科学》2011年第5期1-3,7,共4页Shandong Agricultural Sciences

基　　金：国家转基因专项(2008ZX08001-001)

摘　　要：稻纵卷叶螟等鳞翅目害虫对cry2A*编码的Bt毒性蛋白有明显的排斥效应,从而可达到抗虫的效果。本研究利用农杆菌介导法,将载有cry2A*基因的pC-2A质粒转入受体水稻品种镇稻88基因组内,获得转基因植株。由于cry2A*与抗Basta基因紧密连锁,通过Basta抗性筛选,进而得到转cry2A*基因阳性株。PCR和AGE分析结果表明,外源基因cry2A*已成功整合到镇稻88基因组内。田间试验结果显示转基因阳性植株有明显的抗虫效果。Lepidopteran pests,especially stemborers and leaffolders,had obvious repulsion to Bacillus thuringiensis（BT） toxic protein encoded by cry2A＊ gene,so the pC-2A plasmid with cry2A＊ gene was transferred into the genome of rice variety Zhendao 88 by Agrobacterium-mediated method.Then the transgenic rice plants were obtained through Basta screening based on the tight linkage of cry2A＊ gene with Basta-resistant gene.The PCR and AGE analysis indicated that the cry2A＊ gene had been successfully integrated into Zhendao 88 genome.The field test showed that the transgenic plants had much better insect resistance compared with common plants.

关 键 词：农杆菌介导法 cry2A＊基因 水稻 抗虫性 

分 类 号：S511.035.3[农业科学—作物学]