民猪PRLR基因PCR-SSCP多态性与产仔数关联分析  

The Association Analysis Between PRLR Gene PCR-SSCP Polymorphism and Litter Size in Min Pigs

作　　者：黄贺[1,2] 狄生伟[2] 田亚光[2] 王希彪[2] 李和平[1] 张贵学[2] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《中国农业科学》2011年第11期2341-2346,共6页Scientia Agricultura Sinica

基　　金：国家科技支撑计划项目(2008BADB2B02-5)

摘　　要：【目的】研究民猪促乳素受体基因PRLR的多态性与母猪产仔数的相关性。【方法】采用PCR-SSCP技术检测民猪PRLR基因多态性,最小二乘法分析其多态性对母猪产仔数影响的遗传效应。【结果】PRLR基因在民猪中存在多态性,B等位基因为优势等位基因;在PRLR-1座位,对于初产母猪,BB基因型母猪的TNB和NBA比AA基因型母猪分别多0.93头和0.78头(P<0.05);对于经产母猪,BB基因型母猪的TNB和NBA比AA基因型母猪分别多0.62头和0.74头(P<0.05)。在PRLR-2座位,对于初产和经产母猪,3种基因型个体的TNB之间和NBA之间差异不显著(P>0.05)。在民猪群中这两个座位均处于Hardy-Weinberg平衡状态。【结论】PRLR基因B等位基因对民猪产仔数性状有显著影响。【Objective】The effect of polymorphism in PRLR gene on litter size in Min Pigs was studied.【Method】The polymorphism of PRLR gene was investgated by PCR-SSCP method and the effect of PRLR gene on litter size traits in Min Pigs was analyzed using the least square analysis.【Result】The results showed that the polymorphism in PRLR gene was found.B allele was the dominant allele.BB genotypic sow of Min Pigs produced 0.93 TNB and 0.78 NBA more than AA genotypic sow on the locus of PRLR-1 for the first parity,respectively（P0.05）,0.62 TNB and 0.74 NBA for later parities（P0.05）.TNB and NBA had no significant difference among three kinds of genotype on the locus of PRLR-2 for both the first parity and later parities（P0.05）.Min Pigs are in the situation of Hardy-Weinberg balance on both locus.【Conclusion】The effect of B allele of PRLR gene on litter size is significant.

关 键 词：PRLR基因 多态性 繁殖性能 民猪 SSCP 

分 类 号：S828[农业科学—畜牧学]