地方品种皖南黄肉种鸡ALV-J与REV的共感染及其分子变异分析  

Identification of Co-Infection of ALV-J and REV and Molecular Characterization of the Two Viruses Isolated from Wannan Yellow Feather Broilers

作　　者：张振杰[1] 刘绍琼[1] 王波[1] 崔治中[1,2] 张永光[3] 孙淑红[1,2] 

机构地区：[1]山东农业大学动物医学院,山东泰安271018 [2]山东省畜禽疫病防治工程技术研究中心,山东泰安271018 [3]中国农业科学院兰州兽医研究所,兰州730046

出　　处：《中国农业科学》2011年第11期2379-2386,共8页Scientia Agricultura Sinica

基　　金：家畜疫病病原体生物学国家重点实验室开放课题(SKLVEB2010KFKT001);国家公益性行业(农业)科研专项(200803019)

摘　　要：【目的】探讨地方品种皖南黄肉种鸡群中禽白血病病毒(ALV)与禽网状内皮增生病病毒(REV)的感染状态,同时对J亚型ALV(ALV-J)分离株的囊膜糖蛋白基因(env)和非编码区3′UTR序列进行分子变异分析,并对近十年的分离株非保守序列区段进行统计比较,探讨ALV-J分子变异趋势。【方法】取7只300日龄皖南黄肿瘤病鸡分别进行病理组织学观察、IFA检测肝脏组织切片后激光共聚焦观察、细胞培养后的IFA以及PCR方法检测。【结果】7只皖南黄肉种鸡有6只同时感染ALV-J与REV,而且ALV-J和REV已经共感染同一个肝细胞。取2个ALV-J分离株进行env基因与非编码区3′UTR扩增与序列分析,两分离株的env全长均为1 704 bp,3′UTR全长均为400 bp,3′UTR区段rTM和E元件同发生双缺失;3′UTR-E元件与近十年国内来自白羽肉鸡的ALV-J分离株比较,基因缺失表现为共性,但3′UTR-rTM基因缺失呈现多样性;5′LTR作为保守序列较之前所有国内外毒株有11 bp缺失。【结论】研究发现,地方品种皖南黄肉种鸡群存在ALV-J与REV的共感染,且呈现普遍性(6/7),表明地方品种鸡高肿瘤发生率与ALV-J、REV共感染密切相关;ALV-J的基因缺失主要集中于3′UTR-E与3′UTR-rTM,3′UTR-E元件基因缺失显示,皖南黄肉种鸡群ALV-J分离株与近十年国内来自白羽肉鸡的ALV-J分离株有共同的来源。The objective of the study is to explore co-infection of reticuloendothelial hyperplasia virus（REV） and avian leukemia virus（ALV） in Wannan yellow feather broilers.In addition,the sequence variations in the envelope glycoprotein gene（env） and the 3′UTR and the 5′LTR non-coding sequences of ALV-J collected in the last decade were analyzed.Seven 300-d old chickens suffering from neoplastic diseases were subjected to histopathology examination.Cell cultures were established for viral detection and quantification using PCR and IFA.The result showed that 6 of the 7 animals were co-infected with both viruses,while ALV-A and ALV-B were not detected.Furthermore,both viruses were detected in the same liver cells.Sequence analysis showed that the full-length of the env gene and the 3′UTR region were 1 704 bp and 400 bp,respectively,and both the rTM and the E elements in the 3′UTR region were deleted.Sequence comparison showed that the deletion of the 3′UTR-E element of ALV-J from Wannan yellow-feather broilers is also shared among the ALV-J isolates collected by the authors from the white-feather broilers in the past decade,whereas the deletion of the 3′UTR-rTM element was variable.The 5′LTR conserved sequence had an 11 bp deletion compared to the previously analyzed strains.The remarkably high frequency（6/7） of REV and ALV-J co-infection in association with the high tumor occurrence in Wannan yellow-feather chickens suggest that such co-infection may be an important contributing factor for the tumor epidemic.The fact that the deletion in the 3′UTR-E element is shared by the ALV-J isolates from both Wannan yellow-feather broilers and the white-feather broilers indicates that these ALV-J isolates are of a common origin.

关 键 词：皖南黄肉种鸡 J亚群禽白血病病毒 共感染 遗传变异 

分 类 号：S858.31[农业科学—临床兽医学]